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Anti rabbit antibodies

Manufactured by Merck Group
Sourced in United States

Anti-rabbit antibodies are laboratory reagents used to detect and quantify the presence of rabbit proteins in biological samples. They are designed to specifically bind to rabbit-derived proteins, enabling researchers to analyze and measure their abundance in various experimental settings.

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2 protocols using anti rabbit antibodies

1

Reagents and Antibodies for Cell Assays

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Culture media, fetal bovine serum, penicillin-streptomycin solution, glutamine, DMSO, and sulforhodamine B (SRB) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Muse kits were obtained from EMD Millipore Bioscience (Billerica, MA, USA). 6-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) was from Molecular Probes (Warsaw, Poland). The antibodies against AKT and phospho-(Ser473)-AKT were from Santa Cruz Biotechnology (Heidelberg, Germany). The PARP antibody was from Cell Signaling Technology (Danvers, MA). Anti-β-actin and anti-rabbit antibodies were from Sigma–Aldrich.
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2

Investigating STAT and MAPK Signaling Pathways

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Rat GH, HRP-conjugated goat anti-mouse, and anti-rabbit antibodies were obtained from Sigma (USA). Total STAT1 and phospho-STAT1 (Tyr701), total STAT3 and phospho-STAT3 (Tyr705), total STAT5 and phospho-STAT5 (Tyr694), total ERK1/2 and phospho-ERK1/2 (Thr202/Tyr204), and total JAK2 and phospho-JAK2 (Tyr1007/1008) were obtained from Cell Signaling Technology (USA). The enhanced chemiluminescence (ECL) and Difco skim milk were purchased from Pierce (USA). Polyvinylidene fluoride (PVDF) membranes were obtained from Millipore (Bedford, MA, USA). The above-mentioned antibodies and reagents were used for Western blot analysis. Anti-GHR antibody and Fluorescein isothiocyanate (FITC) were obtained from Sigma (USA) and used for indirect immunofluorescence assay. Unless otherwise stated, other reagents were purchased from Sigma-Aldrich (USA). The above-mentioned reagents were used for cell culture.
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