Hs00797944 s1

Manufactured by Thermo Fisher Scientific

Sourced in Switzerland

Hs00797944_s1 is a pre-designed TaqMan Gene Expression Assay provided by Thermo Fisher Scientific. It is intended to be used for the quantification of a specific gene target in a sample using real-time PCR technology.

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Lab products found in correlation

Hs00215872 m1, by Thermo Fisher Scientific (2 mentions) Mircury rna isolation kit, by Qiagen (1 mentions) Abi prism 7500 sequence detection system, by Thermo Fisher Scientific (1 mentions) Mm00443258 m1, by Thermo Fisher Scientific (1 mentions) Mm00516023 m1, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Hs00174103 m1, by Thermo Fisher Scientific (1 mentions) Hs00174128 m1, by Thermo Fisher Scientific (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using hs00797944 s1

RNA Isolation and RT-PCR Quantification

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The miRCURY RNA Isolation Kit from Exiqon was used for RNA extraction as per the manufacturer’s instructions. RT-PCR was performed as previously described [10 (link)]. The gene expression assays Hs00215872_m1 and Hs00797944_s1 (Applied Biosystems, Rotkreuz, Switzerland) were used to quantitatively measure mRNA of WIPI1 (WD repeat domain phosphoinositide-interacting protein 1) and LC3B, respectively. HMBS was included in analysis as a housekeeping gene for normalization and primers and probes were used as previously published [11 (link)]. The ABI PRISM 7500 Sequence Detection System (Applied Biosystems, Rotkreuz, Switzerland) was used to perform measurements.

Adams O., Janser F.A., Dislich B., Berezowska S., Humbert M., Seiler C.A., Kroell D., Slotta-Huspenina J., Feith M., Ott K., Tschan M.P, & Langer R. (2018). A specific expression profile of LC3B and p62 is associated with nonresponse to neoadjuvant chemotherapy in esophageal adenocarcinomas. PLoS ONE, 13(6), e0197610.

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HDL Modulates Inflammatory Responses in T84 Cells

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The T84 cells were incubated with HDL 50–200 μg/mL for 18 hours and then with tumor necrosis factor (TNF) 25 ng/mL for 3 hours. Total RNA was isolated using TRIzol reagent (Invitrogen). We reverse transcribed 1–2 μg RNA with a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Rotkreuz, Switzerland) before real-time polymerase chain reaction (RT-PCR) analysis (7900HT; Applied Biosystems) using various TaqMan assays: Hs00174128_m1 (human TNF), Mm00443258_m1 (murine TNF), Hs00174103_m1 (human interleukin-8 [IL-8]), Hs00164932_m1 (human intracellular adhesion molecule [ICAM]), Mm00516023_m1 (murine ICAM), Hs00222677_m1 (human β-actin), 4352341E_mACTB (murine β-actin), Hs00797944_s1 (LC3), Hs00250530_m1 (ATG16L1), and endogenous controls for human and animals (Applied Biosystems). Constitutively expressed β-actin was measured as an internal standard for normalization. Relative mRNA levels were calculated using the comparative threshold cycle method. For each experiment, all tests were performed in triplicate. The mRNA levels obtained in control conditions were set to 1, and the results are shown relative to those.

Gerster R., Eloranta J.J., Hausmann M., Ruiz P.A., Cosin-Roger J., Terhalle A., Ziegler U., Kullak-Ublick G.A., von Eckardstein A, & Rogler G. (2014). Anti-inflammatory Function of High-Density Lipoproteins via Autophagy of IκB Kinase. Cellular and Molecular Gastroenterology and Hepatology, 1(2), 171-187.e1.

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Gene Expression Profiling of WIPI1 and MAP1LC3B

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RNA extraction, RT-PCR and real-time qRT-PCR (qPCR) and data analysis were performed as described.^{11 (link)} Gene Expression Assay for WIPI1 and MAP1LC3B (microtubule-associated protein 1 light chain 3) used in a 96-well format on the StepOne plus sequence detection system were Hs00215872_m1 and Hs00797944_s1, respectively (Applied Biosystems, Rotkreuz, Switzerland). HMBSABL1 primers where 5′-TGGAGATAACACTCTAAGCATAACTAAAGGT-3′ and 5′-GATGTAGTTGCTTGGGACCCA-3′ and probe was 5′-FAM-CCATTTTTGGTTTGGGCTTCACACCATT-TAMRA-3′.

Brigger D., Schläfli A.M., Garattini E, & Tschan M.P. (2015). Activation of RARα induces autophagy in SKBR3 breast cancer cells and depletion of key autophagy genes enhances ATRA toxicity. Cell Death & Disease, 6(8), e1861-.

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