Ventana eber 1 dnp probe

In order select EBV-positive AIL samples, the EBV was detected by in situ hybridization (ISH). The formalin-fixed paraffin-embedded (FFPE) tissue sections used for this detection were initially deparaffinized, then rehydrated in a graded solution of xylene and alcohol and deproteinized with proteinase K (ThermoFisher Scientific, Illkirch-Graffenstaden, France). They were subsequently incubated with the Ventana EBER 1 DNP Probe^® (Roche Diagnostics, Meylan, France; catalog number 760-1209) used for EBER hybridization, followed by staining with the Ventana ISH iVIEW blue detection kit^® (Roche Diagnostics; catalog number 760-097). Images of the EBER staining obtained for control 1 (absence of EBER detection) and for a patient with AITL are visible in the Figure S1.

Detection of EBER1 by ISH was used to determine the presence of EBV allowing sample selection. Briefly, the formalin-fixed paraffin-embedded (FFPE) tissue sections were deparaffinized, rehydrated in a graded solution of xylene and alcohol, then deproteinized with proteinase K before incubation with the Ventana EBER 1 DNP Probe^® (Roche Diagnostics, Meylan, France; catalog number 760-1209) on the Benchmark XT automaton^TM (Roche Diagnostics). This was followed by staining with Ventana ISH iVIEW blue plus detection kit^® (Roche Diagnostics; catalog number 760-097) on the same apparatus.
For EBV positive samples, further work was performed on frozen material collected at the same time as the FFPE tissue.