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2 protocols using antibodies against p21 sc 397

1

Antibody-based Protein Quantification Assay

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Antibody against ESM1 (ab103590) was purchased from Abcam (Cambridge. MA). Antibodies against p21 (sc-397; 1:1000), MMP-2 (sc-53630; 1:1000), MMP-9 (sc-6840; 1:1000), uPA (sc-14019; 1:1000), cyclin D1 (sc717; 1:1000), and β-actin (sc-47778; 1:2000) were purchased from Santa Cruz Biotechnology (Dallas, Texas). Horseradish peroxidase conjugated anti-mouse and anti-rabbit secondary antibodies were obtained from Promega (Madison, WI). MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] was purchased from Sigma (St. Louis, MO). Recombinant Human TIMP-1 Protein was purchased from R&D Systems, Inc (Minneapolis, MN). Antibody against TIMP-1(#8946; 1:1000) was purchased from Cell Signaling Technology Inc. (Beverly, MA, USA).
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2

Antibody Procurement for Protein Analysis

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Anti-GSTM1 (GTX113448), anti-β-actin (GTX109639) and anti-α-tubulin (GTX112141) antibodies were purchased from GeneTex (Taichung, Taiwan). Anti-NAD(P)H quinone oxidoreductase-1 (NQO1) (ab2346) was purchased from Abcam (Cambridge, UK). Anti-transcription factor Sp1 (Sp1) (07–645) was purchased from Millipore (Billerica, MA, USA). Anti-GSTO1 (15124-1-AP) was purchased from Proteintech (Chicago, IL, USA). Antibodies against p21 (sc-397) and cyclic AMP-dependent transcription factor ATF-2 (ATF2) (sc-6233) were purchased from Santa Cruz (Dallas, Texas, USA). Peroxidase-conjugated secondary antibodies were purchased from Jackson ImmunoResearch (West Grove, PA, USA).
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