Fitc conjugated annexin 5 and annexin 5 binding buffer

Manufactured by BD

FITC-conjugated Annexin V is a fluorescent-labeled protein that binds to phosphatidylserine (PS) exposed on the cell surface during apoptosis. Annexin V binding buffer is a solution used to facilitate the binding of FITC-conjugated Annexin V to PS.

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Lab products found in correlation

Fortessa 5 flow cytometer, by BD (2 mentions) Celltiter 96 non radioactive cell proliferation assay, by Promega (2 mentions)

Close spelling variants of this product

Annexin V-FITC binding buffer Annexin V binding buffer FITC-conjugated Annexin V Annexin binding buffer Annexin V binding Annexin V buffer Annexin V-FITC Annexin V (Annexin V-FITC) Binding buffer Annexin buffer

2 protocols using fitc conjugated annexin 5 and annexin 5 binding buffer

Cell Proliferation and Apoptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell proliferation was determined by a CellTiter 96^® Non-Radioactive Cell Proliferation Assay (Promega) kit. Absorbance at 490 nm was measured on a Biotek PowerWave (Biotek, USA) plate scanner to obtain raw data. Average absorbance from triplicate wells were plotted against concentration using GraphPad Prism software v.7.0 (GraphPad software). At the indicated time points, cells were stained using FITC-conjugated Annexin V and Annexin V binding buffer (BD Pharmingen^™) according to the manufacturer’s instructions. Annexin V or propidium iodide (PI)-positive cells were analyzed using a Fortessa 5 Flow Cytometer (Becton Dickinson) and data was analyzed using FlowJo^® software (Tree Star).

Li Y., Lim C., Dismuke T., Malawsky D.S., Oasa S., Bruce Z.C., Offenhäuser C., Baumgartner U., D’Souza R.C., Edwards S.L., French J.D., Ock L.S., Nair S., Sivakumaran H., Harris L., Tikunov A.P., Hwang D., Del Mar Alicea Pauneto C., Maybury M., Hassall T., Wainwright B., Kesari S., Stein G., Piper M., Johns T.G., Sokolsky-Papkov M., Terenius L., Vukojević V., Gershon T.R, & Day B.W. (2023). Preventing recurrence in Sonic Hedgehog Subgroup Medulloblastoma using the OLIG2 inhibitor CT-179. Research Square.

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Cell Proliferation and Apoptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell proliferation was determined by a CellTiter 96 ® Non-Radioactive Cell Proliferation Assay (Promega) kit. Absorbance at 490 nm was measured on a Biotek PowerWave (Biotek, USA) plate scanner to obtain raw data. Average absorbance from triplicate wells were plotted against concentration using GraphPad Prism software v.7.0 (GraphPad software). At the indicated time points, cells were stained using FITC-conjugated Annexin V and Annexin V binding buffer (BD Pharmingen TM ) according to the manufacturer's instructions. Annexin V or propidium iodide (PI)positive cells were analysed using a Fortessa 5 Flow Cytometer (Becton Dickinson) and data was analysed using FlowJo ® software (Tree Star).

Li Y., Dismuke T., Lim C., Bruce Z.C., Offenhäuser C., Baumgartner U., Maybury M., D’Souza R.C.J., Hassall T., Wainwright B.J., Stein G., Piper M., Johns T.G., Sokolsky‐Papkov M., Gershon T.R., & Day B.W. (2022). Targeting OLIG2 increases therapeutic responses in SHH medulloblastoma mouse models and patient-derived medulloblastoma organoids.

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