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Mouse cd11b antibody

Manufactured by BioLegend
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The Mouse CD11b Antibody is a monoclonal antibody that binds to the CD11b protein, also known as the integrin alpha M chain or Mac-1 alpha chain. CD11b is expressed on the surface of various immune cells, including monocytes, macrophages, and granulocytes. This antibody can be used to identify and study these cell types in mouse models and research applications.

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Lab products found in correlation

Htlr4, by InvivoGen (2 mentions) Hek blue detection, by InvivoGen (2 mentions) Hek blue selection, by InvivoGen (2 mentions) Ack lysis buffer, by Thermo Fisher Scientific (2 mentions) Facsaria 2, by BD (2 mentions)

2 protocols using mouse cd11b antibody

1

Quantification of Cytosolic LPS in Splenocytes

Cited 1 time
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Cytosolic fraction was isolated as previously described (Vanaja et al., 2016 (link)). Spleens were collected 5 h post-LPS injection and crushed through 100 μm nylon-mesh strainer. RBCs were lysed using ACK lysis buffer (Gibco). Splenocytes were incubated with Fc block, stained with mouse CD11b antibody (Clone M1/70; BioLegend catalog no. 101212) and sorted on BD FACS Aria II. Sorted CD11b + cells were resuspended in 0.001% digitonin buffer for 8 min and supernatant containing the cytosolic content was collected. Cytosolic LPS was quantified using the Limulus Amebocyte Lysate (LAL) assay (Associates of Cape Cod) according to the manufacturer’s instructions. HEK293 cells expressing hTLR4 (Invivogen) were cultured with HEK-Blue Detection and HEK-Blue Selection (Invivogen) in a 96-well plate according to the manufacturer’s protocol. Cytosol from CD11b+ splenocytes was added to the cell culture medium and SEAP activity was assessed by measuring absorbance at 620–655 nm.
Vasudevan S.O., Russo A.J., Kumari P., Vanaja S.K, & Rathinam V.A. (2022). A TLR4-independent critical role for CD14 in intracellular LPS sensing. Cell reports, 39(5), 110755.
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2

Quantification of Cytosolic LPS in Splenocytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytosolic fraction was isolated as previously described (Vanaja et al., 2016 (link)). Spleens were collected 5 h post-LPS injection and crushed through 100 μm nylon-mesh strainer. RBCs were lysed using ACK lysis buffer (Gibco). Splenocytes were incubated with Fc block, stained with mouse CD11b antibody (Clone M1/70; BioLegend catalog no. 101212) and sorted on BD FACS Aria II. Sorted CD11b + cells were resuspended in 0.001% digitonin buffer for 8 min and supernatant containing the cytosolic content was collected. Cytosolic LPS was quantified using the Limulus Amebocyte Lysate (LAL) assay (Associates of Cape Cod) according to the manufacturer’s instructions. HEK293 cells expressing hTLR4 (Invivogen) were cultured with HEK-Blue Detection and HEK-Blue Selection (Invivogen) in a 96-well plate according to the manufacturer’s protocol. Cytosol from CD11b+ splenocytes was added to the cell culture medium and SEAP activity was assessed by measuring absorbance at 620–655 nm.
Vasudevan S.O., Russo A.J., Kumari P., Vanaja S.K, & Rathinam V.A. (2022). A TLR4-independent critical role for CD14 in intracellular LPS sensing. Cell reports, 39(5), 110755.
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