Anti prpa32 s33

Manufactured by Fortis Life Sciences

Sourced in United States

The Anti-pRPA32 S33 is a laboratory product designed to detect the phosphorylation of the RPA32 subunit at serine 33. This antibody can be used in various immunoassay techniques to identify and quantify the phosphorylated form of this protein.

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Lab products found in correlation

Anti rpa32, by Fortis Life Sciences (3 mentions) Anti xpc, by Fortis Life Sciences (2 mentions) Anti h2ak119ub1, by Fortis Life Sciences (2 mentions) Anti lamin b, by Fortis Life Sciences (2 mentions) Anti xpa, by Fortis Life Sciences (2 mentions) Anti phospho chk1 s345, by Fortis Life Sciences (2 mentions) Anti ring1a, by Abcam (2 mentions) Anti rpa32, by Novus Biologicals (2 mentions) Anti rad51, by Novus Biologicals (2 mentions) Anti h2ak119ub1, by Merck Group (2 mentions) Alexa conjugate, by Merck Group (2 mentions) Azd1775, by Selleck Chemicals (1 mentions) Hrp conjugated goat anti rabbit, by Cell Signaling Technology (1 mentions) Anti rpa32, by Abcam (1 mentions) Anti prpa32 s4 s8, by Fortis Life Sciences (1 mentions) Olaparib, by Selleck Chemicals (1 mentions) Anti mouse antibodies, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti actin, by Merck Group (1 mentions) Anti rad51, by Abcam (1 mentions)

Close spelling variants of this product

PRPA32 (S33, PRPA32

4 protocols using anti prpa32 s33

Combinatorial treatment with AZD1775 and olaparib

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AZD1775 and olaparib were purchased from Selleck Chemicals (Houston, TX). Reagents were formulated and stored according to manufacturer’s protocols for in vitro and in vivo experiments. Following antibodies were used: primary antibodies against caspase 3 (#9664), Bax (#5023), MRE11 (#4847), NBS1 (#14956), ATM (#2873), RAD51 (#8875), 53BP1 (#4937), γH2AX (#9718), cyclinB1 (#12231), CDC2 (#28439), pCDC2 (Y15; #4539), pHH3 (#53348), ATR (#2790), pATR (S428; #2853),Chk1 (#2360), pChk1 (S317; #12302), pChk1(S345, #2348), histone H3 (#4499) and secondary HRP-conjugated goat anti-rabbit (#7074) and anti-mouse antibodies (#7076) from Cell Signal Technology (CST, Danvers, MA); anti-RPA32 (#ab76420) from Abcam (Cambridge, MA); anti-pRPA32 (S4/S8, #A300-245A) and anti-pRPA32 (S33, #A300-246A) from Bethyl Laboratories (Montgomery, TX); anti-PARP1(#Sc-8007) from Santa Cruz (Dallas, TX); anti-PAR (Ab-1, 10H; #AM80) from Merck Millipore, (Darmstadt, Germany); and anti-β-actin (#A5441) from Sigma-Aldrich (St. Louis, MO).

Lin X., Chen D., Zhang C., Zhang X., Li Z., Dong B., Gao J, & Shen L. (2018). Augmented antitumor activity by olaparib plus AZD1775 in gastric cancer through disrupting DNA damage repair pathways and DNA damage checkpoint. Journal of Experimental & Clinical Cancer Research : CR, 37, 129.

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Antibody Validation in DNA Damage Response

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The following antibodies were used in this study: anti-FLAG M2 (Sigma-Aldrich, St Louis, USA), anti-RBM14 (Abclonal, Wuhan, China), anti-Actin (Sigma-Aldrich), anti-BRCA1 (Proteintech, Chicago, USA), anti-RPA70 (Abclonal), anti-GST (MBL, Tokyo, Japan), anti-His (MBL), anti-RPA32 (Bethyl, Montgomery, USA), anti-pRPA32 S33 (Bethyl), anti-ATM (Bethyl), anti-pATM S1981 (Abcam, Cambridge, UK), anti-CldU (Abcam), anti-RAD51 (Abcam), anti-MRE11 (Bethyl), anti-RAD50 (Bethyl), anti-NBS1 (Bethyl), anti-CTIP (Santa Cruz Biotech, Santa Cruz, USA), anti-gamma H2AX (CST, Danvers, USA), fluorescein (FITC)-AffiniPure anti-rabbit IgG (H+L) (Jackson ImmunoResearch, Philadelphia, USA), and Alexa Fluorescein 594-AffiniPure anti-mouse IgG (H+L) (Jackson ImmunoResearch), Peroxidase AffiniPure donkey anti-rabbit IgG (H+L) (Jackson ImmunoResearch), and Peroxidase AffiniPure goat anti-mouse lgG (H+L) (Jackson ImmunoResearch).

Li Z., Liao Y., Tang C., Xu L., Peng B, & Xu X. (2023). RBM14 promotes DNA end resection during homologous recombination repair: RBM14 in HR repair. Acta Biochimica et Biophysica Sinica, 55(12), 1864-1873.

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Western Blot Analysis of DNA Damage Proteins

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For western blot of endogenous proteins, anti-γH2AX (Cell Signaling Technology (CST), MA #9718, 1:1000), anti-Actin B (CST, MA #4970, 1:1000), anti-p-ATM S1981 (CST, MA #13050, 1:1000), anti-total ATM (CST, MA #2873, 1:1000), anti-total H2AX (CST, MA #2595, 1:1000), anti-RING1A (CST, MA #13069, 1:1000), anti-RING1B (Santa Cruz (SC), CA sc-101109, 1:1000), anti-H2AK119ub1 (CST, MA #8240,1:1000), anti-XPC (SC, CA sc-74410, 1:1000), anti-lamin B (SC, CA sc-6216, 1:1000; SC, CA sc-374015, 1:1000), anti-XPA (SC, CA sc-28353, 1:1000), anti-pRPA32 S33 (Bethyl Laboratories, TX A300–246A-M, 1:1000), anti-RPA32 (CST, MA #2208,1:1000), anti-phospho-Chk1 S345 (CST, MA #2348, 1:1000) and anti-total Chk1 (CST, MA #2360, 1:1000) antibodies were used. For immunofluorescence, anti- γH2AX (CST,MA #9718, 1:100), anti-RING1A (Abcam, CA ab175149, 1:100), anti-RPA32 (CST, MA #2208, 1:100), anti-Rad51 (Novus biological, CO NB100–148, 1:100), anti-H2AK119ub1 (Millipore Sigma, MA 05–678,1:100) and secondary Alexa Conjugate (CST, MA rat #4416, 1:1000, mouse #8890, 1:500, rabbit #4412, 1:1000 and, rabbit #8889,1:500) antibodies were used.

Sriramkumar S., Matthews T.D., Ghobashi A.H., Miller S.A., VanderVere-Carozza P.S., Pawelczak K.S., Nephew K.P., Turchi J.J, & O’Hagan H.M. (2020). Platinum-Induced Ubiquitination of Phosphorylated H2AX by RING1A is Mediated by Replication Protein A in Ovarian Cancer. Molecular cancer research : MCR, 18(11), 1699-1710.

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Comprehensive Western Blot and Immunofluorescence Analysis of DNA Damage Response Proteins

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For western blot of endogenous proteins, anti-gH2AX (Cell Signaling Technology (CST), MA #9718, 1:1000), anti-Actin B (CST, MA #4970, 1:1000), anti-p-ATM S1981 (CST, MA #13050, 1:1000), anti-total ATM (CST, MA #2873, 1:1000), anti-total H2AX (CST, MA #2595, 1:1000), anti-RING1A (CST, MA #13069, 1:1000), anti-RING1B (Santa Cruz (SC), CA sc-101109, 1:1000), anti-H2AK119ub1 (CST, MA #8240,1:1000), anti-XPC ( SC, CA sc-74410, 1:1000), anti-lamin B (SC, CA sc-6216, 1:1000 ; SC, CA sc-374015, 1:1000), anti-XPA (SC, CA sc-28353, 1:1000), anti-pRPA32 S33 (Bethyl Laboratories, TX A300-246A-M, 1:1000), anti-RPA32 (CST, MA #2208,1:1000), antiphospho-Chk1 S345 (CST, MA #2348, 1:1000) and anti-total Chk1 (CST, MA #2360, 1:1000) antibodies were used. For immunofluorescence, anti-gH2AX (CST,MA #9718, 1:100), anti-RING1A (Abcam, CA ab175149, 1:100), anti-RPA32 (CST, MA #2208, 1:100), anti-Rad51 (Novus biological, CO NB100-148, 1:100), anti-H2AK119ub1 (Millipore Sigma, MA 05-678,1:100) and secondary Alexa Conjugate (CST, MA rat #4416, 1:1000, mouse #8890, 1:500, rabbit #4412, 1:1000 and, rabbit #8889,1:500) antibodies were used.

Sriramkumar S., Matthews T.D., Ghobashi A.H., Miller S.A., VanderVere-Carozza P.S., Pawelczak K.S., Nephew K.P., Turchi J.J., & O’Hagan H.M. (2020). Platinum-Induced Ubiquitination of Phosphorylated H2AX by RING1A is Mediated by Replication Protein A in Ovarian Cancer.

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