Adiponectin

Manufactured by Bioss Antibodies

Sourced in China

Adiponectin is a protein that plays a role in regulating glucose and lipid metabolism. It is produced and secreted by adipose tissue and has anti-inflammatory and insulin-sensitizing properties.

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Lab products found in correlation

C ebpα, by Bioss Antibodies (2 mentions) Pparγ, by Bioss Antibodies (2 mentions)

2 protocols using adiponectin

Adipose Tissue and Capillary Formation

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The excised neo-tissue samples from the silicone tubes were fixed in 10% formalin, dehydrated with a graded alcohol series, and embedded in paraffin. Sections with a thickness of 4 μm were stained with hematoxylin and eosin (H&E). Images of the histologic sections were examined microscopically (Leica, Germany) at a magnification of ×100. To quantitatively analyze the area of intact adipocytes and the number of mature capillaries, images in 8 random fields per section from each group (×100 magnification) were examined by using ImageJ software. Immunochemical staining for C/EBP-α (Bioss, China, cat.bs1630R), PPARγ (Bioss, China, cat.bs4888R), Adiponectin (Bioss, China, cat.bs0471R), and CD31 (Sino Biological, China, cat.50408-T16) was performed to determine the extent of adipose tissue and blood vessel formation in new growth tissues at the 4th week.

Hong P., Xu X., Hu X., Yang H., Wu Y., Chen J., Li K, & Tang Z. (2021). Therapeutic potential of small extracellular vesicles derived from lipoma tissue in adipose tissue regeneration—an in vitro and in vivo study. Stem Cell Research & Therapy, 12, 222.

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Histological Analysis of Adipose Tissue

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The excised neo-tissue samples from the silicone tubes were xed in 10% formalin, dehydrated with a graded alcohol series, embedded in para n. Sections with a thickness of 4 µm were stained with hematoxylin and eosin (H&E). Images of the histologic sections were examined microscopically (Leica, Germany) at magni cation of 100×. To quantitatively analyze the area of intact adipocytes and the number of mature capillaries, images in 8 random elds per section from each group (100× magni cation) were examined by using ImageJ software. Immunochemical staining for C/EBP-α (Bioss, China, cat.bs1630R), PPARγ (Bioss, China, cat.bs4888R), Adiponectin (Bioss, China, cat.bs0471R) and CD31 (Sino Biological, China, cat.50408-T16) was performed to determine the extent of adipose tissue and blood vessel formation in new growth tissues on the 4th week.

Hong P., Xu X., Hu X., Yang H., Wu Y., Chen J., Li K., & Tang Z. (2021). Therapeutic Potential of Small Extracellular Vesicles Derived from Lipoma Tissue in Adipose Tissue Regeneration – An in Vitro and in Vivo Study.

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