Empower tm 3 chromatography data software

The identification and quantification of individual phenolic compounds were conducted by chromatographic analysis using the ACQUITY UPLC H-Class system coupled to an ACQUITY UPLC Photodiode Array (PDA) detector. The system was controlled by EmpowerTM 3 Chromatography Data Software (Waters Corporation, Milford, MA, USA).
The PDA detector was set at the wavelength range 200-400 nm for the 3D scan, with a data collection rate of 40 pts s -1 to identify the compounds. A certain wavelength of 2D scans PDA detector (260, 280, and 320 nm), on the basis of the maximum absorbance wavelength with a data collection rate of 80 pts s -1 was used for compound quantification. A binary solvent system was used as the mobile phase. Solvent A was 0.01% acetic acid in water and solvent B was 2% acetic acid in acetonitrile. The analysis was run in 10 min using the following gradient program (%B): 0-0.3 min, 3.1-9.5%; 0.3-0.8 min, 9.5-15.6%; 0.8-5 min, 15.6-82.2%; 5-6 min, 82.2-100%; 6-9 min, 100%; 9-10 min, 3.1%. The flow rate was set at 0.64 mL min -1 [28] (link). Phenolic compounds found in the Roselle extract were identified by comparing the retention time and spectra to those of standards. Addition%ally, a spiking procedure with corresponding standards was performed to confirm the identification.