Luria-Bertani broth and agar were purchased from Difco Laboratories (USA). Chemical re-agents were mainly purchased from Merck (Germany). Glacial acetic acid and ethanol (96%) were prepared from Mojallali co. (Iran). Kanamycin, ampicillin and anti-His tag antibody were purchased from Roche (Germany). Isopropyl β-D-1-thiogalactopyranoside (IPTG) was prepared from SinaClon (Iran). PCR master mix and 1 kb DNA Ladder were purchased from GoldBio(China).
1 kb dna ladder
Manufactured by GoldBio
Sourced in China
The 1 kb DNA Ladder is a molecular weight marker used for estimating the size of DNA fragments in agarose gel electrophoresis. It consists of a set of DNA fragments of known sizes, typically ranging from 250 base pairs to 10,000 base pairs, which can be used as a reference to determine the sizes of unknown DNA samples.
Automatically generated - may contain errors
Lab products found in correlation
Pcr master mix, by GoldBio (2 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Sinaclon (2 mentions)
Ampicillin, by Roche (1 mentions)
Kanamycin, by Roche (1 mentions)
Anti his tag antibody, by Roche (1 mentions)
Luria bertani broth, by BD (1 mentions)
Prestained protein ladder, by Sinaclon (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Mouse anti his tag, by Merck Group (1 mentions)
Hrp conjugated anti mouse igg antibody, by Merck Group (1 mentions)
Ampicillin, by Merck Group (1 mentions)
Lb agar, by BD (1 mentions)
2 protocols using 1 kb dna ladder
1
Bacterial Growth Media Preparation
2
Protein Expression in Bacterial Hosts
Check if the same lab product or an alternative is used in the 5 most similar protocols
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LB (Luria-Bertani) broth and LB agar were prepared from Difco Laboratories (USA). Mouse anti-His tag and HRP-conjugated anti-mouse IgG antibodies were purchased from Sigma (Germany). PCR master mix, as well as 1 kb DNA Ladder, was prepared from GoldBio (China). Ethanol and glacial acetic acid were purchased from Mojallali co. (Iran). Antibiotics (kanamycin and ampicillin) were purchased from Sigma (Germany). Prestained protein ladder and Isopropyl β-D-1-thiogalactopyranoside (IPTG) were purchased from SinaClon (Iran). Bacillus subtilis strain PY79 (ATCC1609) was provided by Iranian Research Organization for Science and Technology, Tehran, Iran. E. coli BL21 (DE3) star was purchased from Royan Institute, Tehran, Iran.
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