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Anti mouse cd45 apc cy7 antibodies

Manufactured by BioLegend
Sourced in United States

Anti-mouse CD45-APC-Cy7 antibodies are a fluorochrome-conjugated antibody product used for the detection and analysis of mouse CD45-expressing cells by flow cytometry. The APC-Cy7 fluorescent dye is coupled to the anti-mouse CD45 antibody, enabling the identification and quantification of CD45-positive cells in mouse samples.

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2 protocols using anti mouse cd45 apc cy7 antibodies

1

Xenograft Model of Leukemia

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All animal experiments were performed with the approval of Dana-Farber Cancer Institute's Institutional Animal Care and Use Committee. NOD.Cg-Prkdcscid Il2rgtm1Sug/JicTac (NOG) mice were obtained from Taconic Biosciences. Nonirradiated 8- to 12-week-old adult mice were transplanted with previously established PDXs (25 (link)) via tail-vein injection (250,000 cells/mouse). Engraftment of human cells (hCD45+) was analyzed and monitored longitudinally by weekly bleeding to quantify hCD45+ cells in the peripheral blood by flow cytometry with human CD45-PE and anti-mouse CD45-APC-Cy7 antibodies (BioLegend). Mice were monitored closely to detect disease onset, and treatment started when hCD45+ cells were detectable in the peripheral blood. Mice were randomly assigned to either normal or 0.1% VTP-50469 rodent special diet (25 (link)). Mice were bled weekly to monitor leukemia burden as described above and euthanized when showing clinical signs of disease (experimental endpoint). Leukemia cells from a subset of these animals were harvested after 7 days of treatment to perform RNA-seq.
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2

Longitudinal PDX Leukemia Monitoring

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All animal experiments were performed with the approval of Dana-Farber Cancer Institute’s Institutional Animal Care and Use Committee (IACUC). NOD.Cg-PrkdcscidIl2rgtm1Sug/JicTac (NOG) mice were obtained from Taconic Biosciences (Rensselaer, NY, USA). Non-irradiated 8–12 weeks old adult mice were transplanted with previously established patient-derived xenografts (PDXs) (25 (link)) via tail vein injection (250,000 cells/mouse). Engraftment of human cells (hCD45+) was analyzed and monitored longitudinally by weekly bleeding to quantify hCD45+ cells in the peripheral blood by flow cytometry with human CD45-PE and anti-mouse CD45-APC-Cy7 antibodies (Biolegend, San Diego, CA, USA). Mice were monitored closely to detect disease onset and treatment started when hCD45+ cells were detectable in the peripheral blood. Mice were randomly assigned to either normal or 0.1% VTP-50469 rodent special diet (25 (link)). Mice were bled weekly to monitor leukemia burden as described above and euthanized when showing clinical signs of disease (experimental endpoint). Leukemia cells from a subset of these animals were harvested after seven days of treatment to perform RNA-Seq.
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