Mab 5b12

A fluorescent antibody test (FAT) was undertaken as described previously [48 ]. Briefly, brain tissue was excised from the cerebellum and two impression smears were made on a microscope slide. Slides were left to air dry for 5 minutes at room temperature before being fixed in 99–100% acetone, washed, rinsed, and stained using FITC anti-rabies monoclonal globulin (Fujirebio, 800-092). Fluorescent green foci would indicate the presence of the N protein thereby indicating virus infection. Positive control material consisted of previously extracted CVS-infected mouse brain. Two mice from each group were tested.
Histopathology and immunohistochemistry were performed as described previously [49 (link)]. Mouse carcasses were fixed in 10% neutral buffered formalin for 2 weeks before being sectioned coronally and processed into paraffin tissue blocks. Serial sections of 4 µm were prepared and stained with haematoxylin and eosin (H&E) for histopathology or immunohistochemistry for rabies virus nucleoprotein using a monoclonal antibody, mAb 5B12 (MyBioSource Inc., San Diego, CA, USA).

At the end of the study, a fluorescent antibody test (FAT) was undertaken, as described previously [43 (link)]. Histopathology and immunohistochemistry were performed, as described previously [44 (link)]. Mouse tissues were fixed in 10% neutral buffered formalin for two weeks before being sectioned coronally and processed into paraffin tissue blocks. Serial sections of 4 µm were prepared and stained with haematoxylin and eosin (H&E) for histopathology or immunohistochemistry for RABV N protein using a monoclonal antibody, mAb 5B12 (MyBioSource Inc., San Diego, CA, USA).