Irdye 800cw conjugated goat anti rabbit igg or anti mouse igg

Manufactured by LI COR

Sourced in United States

IRDye 800CW-conjugated goat anti-rabbit IgG or anti-mouse IgG is a fluorescent labeling reagent. It is used to detect and quantify target proteins in Western blotting, immunohistochemistry, and other protein detection applications.

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Lab products found in correlation

Human ifnα, by Thermo Fisher Scientific (2 mentions) Mouse il 1β, by Thermo Fisher Scientific (2 mentions) Poly 1 c hmw, by InvivoGen (2 mentions) Rb anti myc, by Cell Signaling Technology (2 mentions) Ms anti myc, by Cell Signaling Technology (2 mentions) Rb anti actin, by Merck Group (2 mentions) Ms anti flag, by Merck Group (2 mentions) Rb anti 14 3 3, by Santa Cruz Biotechnology (2 mentions) Ms anti spir 1, by Santa Cruz Biotechnology (2 mentions) Ms anti spir 1, by Abcam (2 mentions) Rb anti ddx3, by Cell Signaling Technology (2 mentions) Rb anti ha, by Merck Group (2 mentions) Ms anti gapdh, by Merck Group (2 mentions) Rb anti irf3, by Cell Signaling Technology (2 mentions) Rb anti phospho irf3 ser396, by Cell Signaling Technology (2 mentions) Irdye 680rd conjugated goat anti rabbit igg or anti mouse igg, by LI COR (2 mentions) Anti c myc agarose, by Santa Cruz Biotechnology (2 mentions) Human tnf α, by Thermo Fisher Scientific (2 mentions) Poly d lysine hydrobromide, by Merck Group (2 mentions) Anti flag m2 affinity gel, by Merck Group (2 mentions)

Close spelling variants of this product

IRDye®800CW anti-rabbit or anti-mouse IgG IRDye 800CW-conjugated goat anti-rabbit IgG IRDye 800CW-conjugated goat anti-mouse IgG IRDye 800CW goat anti-rabbit IgG IRDye 800CW-conjugated goat anti-rabbit IRDye 800CW-conjugated anti-rabbit IgG IRDye 800CW goat anti-mouse IgG IRDye 800CW-conjugated goat anti-mouse IRDye 800CW-conjugated anti-mouse IgG 800CW goat anti-mouse or goat anti-rabbit

3 protocols using irdye 800cw conjugated goat anti rabbit igg or anti mouse igg

Immunoblotting Protocols Using Diverse Antibodies

Cited 1 time

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Primary antibodies used were from the following sources: rabbit (Rb) anti-Myc (Cell Signaling, 2278), mouse (Ms) anti-Myc (Cell Signaling, 9B11), Rb anti-actin (Sigma, A2066), Ms anti-FLAG (Sigma F1804), Rb anti-14-3-3 (Santa Cruz, sc-629), Ms anti -Spir-1 (Santa Cruz, sc-517039), Ms anti-Spir-1 (Abcam, ab57463), Rb anti-DDX3 (Cell Signaling, 2635), Rb anti-IKKβ (Cell Signaling, 2684), Rb anti-HA (Sigma, H6908), Ms anti-α-tubulin (Millipore, 05–829), Ms anti-GAPDH (Sigma, G8795), Rb anti-IRF3 (Cell Signaling, 4962), Rb anti-IRF3 (Santa Cruz, SC-9082), Ms anti-COPε (Santa Cruz, sc-133194), Rb anti-phospho-IRF3 Ser396 (Cell Signaling, 4947S) and Rb polyclonal anti-C6 [53 (link)]. For dilutions used for the primary antibodies, see S3 Table. Secondary antibodies used (1:10,000 dilution) were IRDye 680RD-conjugated goat anti-rabbit IgG or anti-mouse IgG and IRDye 800CW-conjugated goat anti-rabbit IgG or anti-mouse IgG (LI-COR).
Reagents used in this study were: anti-c-Myc agarose from Santa Cruz Biotechnology, and monoclonal anti-HA-agarose, clone HA-7, ANTI-FLAG M2 affinity gel and Poly-D-lysine hydrobromide (all from Sigma Aldrich). Human IFNα, human TNF-α and mouse IL-1β were from Peprotech, HMW poly(I:C) and puromycin were from InvivoGen, and doxycycline was from Melford.

Torres A.A., Macilwee S.L., Rashid A., Cox S.E., Albarnaz J.D., Bonjardim C.A, & Smith G.L. (2022). The actin nucleator Spir-1 is a virus restriction factor that promotes innate immune signalling. PLoS Pathogens, 18(2), e1010277.

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Western Blot Analysis of Apoptosis Markers

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Cells were harvested and lysed in RIPA containing proteinase and phosphatase inhibitors. The concentration of proteins in cell lysates were quantified by BCA kit (Applygen Technologies, China) following the instruction. Equal amounts of cell lysates (20 µg protein/lane) were electrophoresed on SDS-polyacrylamide gels and transferred to nitrocellulose membrane. Membranes were blocked and then probed with the appropriate primary antibody [PYCR1, 1:5,000 (Proteintech); PARP, 1:1,000 (Proteintech); Caspase-3, 1:1,000 (CST); cleaved-Caspase 3, 1:1000 (CST); p-PI3K, 1:2,000 (Proteintech); p-Akt, 1:4,000 (Proteintech); Akt, 1:1,000 (Proteintech); β-actin, 1:8,000 (Applygen)] in blocking buffer overnight at 4 °C. The bound antibodies were detected with IRDye 800CW-conjugated goat anti-rabbit IgG or anti-mouse IgG (at 1:10,000 dilution; LI-COR Bioscience, Lincoln, NE, USA) and visualized with Odyssey 290 infrared imaging system (LI-COR Bioscience).

Xiao S., Li S., Yuan Z, & Zhou L. (2020). Pyrroline-5-carboxylate reductase 1 (PYCR1) upregulation contributes to gastric cancer progression and indicates poor survival outcome. Annals of Translational Medicine, 8(15), 937.

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Antibody Characterization for Immunoblot

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Primary antibodies used were from the following sources: rabbit (Rb) anti-Myc (Cell Signaling, 2278), mouse (Ms) anti-Myc (Cell Signaling, 9B11), Rb anti-Actin (Sigma, A2066), Ms anti-FLAG (Sigma F1804), Rb anti-14-3-3 (Santa Cruz, sc-629), Ms anti -Spir-1 (Santa Cruz, sc-517039), Ms anti-Spir-1 (Abcam, ab57463), Rb anti-DDX3 (Cell Signaling, 2635), Rb anti-IKKb (Cell Signaling, 2684), Rb anti-HA (Sigma, H6908), Ms anti-a-Tubulin (Millipore, 05-829), Ms anti-GAPDH (Sigma, G8795), Rb anti-IRF3 (Cell Signaling, 4962), Ms anti-COPe (Santa Cruz, sc-133194), Rb anti-phospho-IRF3 Ser396 (Cell Signaling, 4947S) and Rb polyclonal anti-C6 (53) . For dilutions used for the primary antibodies, see reagents table. Secondary antibodies used (1:10,000 dilution) were IRDye 680RDconjugated goat anti-rabbit IgG or anti-mouse IgG and IRDye 800CW-conjugated goat anti-rabbit IgG or anti-mouse IgG (LI-COR).
Reagents used in this study were: Anti-c-Myc Agarose from Santa Cruz Biotechnology, and monoclonal Anti-HA-Agarose, clone HA-7, ANTI-FLAG M2 Affinity Gel and Poly-D-lysine hydrobromide (all from Sigma Aldrich). Human IFNα, human TNF-α and mouse IL-1β were from Peprotech, HMW poly(I:C) and puromycin were from InvivoGen, and doxycycline was from Melford.

Torres A.A., Macilwee S., Rashid A., Cox S., Albarnaz J.D., Bonjardim C.A., & Smith G.L. (2020). The actin nucleator Spir-1 is a virus restriction factor that promotes IRF3 activation.

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