Recombinant pdgf bb

The anti-phosphotyrosine (4G10) and anti-c-Abl (OPT20) antibodies were purchased from EMD Millipore (Billerica, MA) and used at 1:1000 and 1:250 dilutions, respectively, for Western blots. Anti-GFP (ab290, used at a 1:500 dilution), anti-tubulin (ab80779, used at a 1:5000 dilution), anti-beta actin (ab8224, used at a 1:5000 dilution), and anti-myc (ab9106, used at a 1:500 dilution) were purchased from Abcam (Cambridge, MA). The GFP-CLASP2α cDNA construct and anti-CLASP2 antibodies were generous gifts from Dr. Anna Akhmanova (Utrecht University, The Netherlands) and used at a 1:250 dilution for Western blots. Recombinant PDGF-BB (Abcam) was used at 100 ng/ml, protein tyrosine phosphatase 1b (PTP1B; Millipore) was used at 1 µg/µl and STI-571 (Novartis, Cambridge, MA) was used at 1 µM. A PDGF receptor inhibitor AG1296 was purchased from EMD Millipore and used at 1 µM final concentration.