7900 ht sequence detection system and sds version 2

Forty paired OSCC tumor and pericancerous normal tissues were homogenized in liquid nitrogen with a mortar and pestle and incubated with RNAzol B reagent (Tel-Test, Friendwood, TX). The RNA was further purified using an RNeasy cleanup kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s protocol. First-strand cDNA was synthesized from 5 μg of total RNA and then mixed with a reaction mixture consisting of commercially available primers (ERAP2 Hs01073631_m1 and normalization control B2M, Hs00984230_m1; Assay-on-Demand, Applied Biosystems, Foster City, CA, USA), RNase-free water, and TaqMan Universal PCR Master Mix. Quantitative RT-PCR was performed and analyzed using a 7900 HT Sequence Detection System and SDS version 2 (Applied Biosystems). All experiments were repeated in duplicate.

The OSCC tumor and normal counterpart tissues were homogenized in liquid nitrogen with a mortar and pestle and incubated with RNAzol B reagent (Tel-Test, Friendwood, TX, USA). The RNA was further purified using a RNeasy cleanup kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s protocol. First-strand cDNA was synthesized from 5 μg of total RNA, and then mixed with a reaction mixture consisting of commercially available primers (RPL36A, Hs01586542_g1 and normalization control ACTB, Hs01060665_g1, Assay-on-Demand, Applied Biosystems, Foster City, CA, USA), RNase-free water, and TaqMan Universal PCR Master Mix. The qPCR was performed and analyzed using a 7900 HT Sequence Detection System and SDS version 2 (Applied Biosystems). All experiments were repeated in duplicate.