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Cleaved casepase3

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Cleaved-caspase-3 is an antibody used to detect the activated form of caspase-3, a key enzyme involved in the apoptosis (programmed cell death) pathway. This antibody recognizes the cleaved, active form of caspase-3, which is a reliable marker of cells undergoing apoptosis.

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Lab products found in correlation

Cleaved parp, by Cell Signaling Technology (2 mentions) Cyclin d1, by Cell Signaling Technology (2 mentions) Bcl 2, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) Medroxyprogesterone acetate, by Abcam (1 mentions) Fatostatin, by Abcam (1 mentions) P nf κb, by Cell Signaling Technology (1 mentions) Nf κb, by Cell Signaling Technology (1 mentions) Akr1c1, by Abcam (1 mentions) Emt kit, by Cell Signaling Technology (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Total actin, by Merck Group (1 mentions) Sod 2, by R&D Systems (1 mentions) Mmp 9, by Abcam (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Chemidoc xrs system, by Bio-Rad (1 mentions) Timp 1, by Abcam (1 mentions)

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Anti-cleaved-casepase3 (2 citations)

3 protocols using cleaved casepase3

1

Investigating Lipogenesis and Cell Cycle Regulation

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For this study, we purchased the following antibodies against SREBP1 (Abcam, ab191857), FASN(Abcam,ab96866), ACLY(Abcam,ab40793), SCD1(Abcam,ab236868), PGR (Abcam, ab32085), Cyclin D1 (Cell Signaling Technologies, #2978), CDK4 (Cell Signaling Technologies, #12790), PARP (Cell Signaling Technologies, #9542), cleaved-PARP (Cell Signaling Technologies, #5625), Bax (Cell Signaling Technologies, #2772), Bcl2 (Cell Signaling Technologies, #4223), NF-κB(Cell Signaling Technologies,#8242), p-NF-κB (Cell Signaling Technologies, #3033), β-actin (Cell Signaling Technologies, #4970), ki67 (Abcam, ab92742), cleaved-casepase3 (Cell Signaling Technologies, #9664), mouse IgG (Cell Signaling Technologies, #7076), and rabbit IgG (Cell Signaling Technologies, #7074). Medroxyprogesterone acetate and Fatostatin were obtained from Abcam and MedChem Express, respectively, and were both diluted in DMSO.
Ma X., Zhao T., Yan H., Guo K., Liu Z., Wei L., Lu W., Qiu C, & Jiang J. (2021). Fatostatin reverses progesterone resistance by inhibiting the SREBP1-NF-κB pathway in endometrial carcinoma. Cell Death & Disease, 12(6), 544.
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2

Immunoblotting and Immunohistochemistry Protocols

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Antibodies used in WB and IHC experiments were following: MGLL (Abcam, ab234701), CDK4 (Cell Signaling Technologies, #12790), Cyclin D1 (Cell Signaling Technologies, #2978), cleaved-PARP (Cell Signaling Technologies, #5625), Bcl2 (Cell Signaling Technologies, #4223), cleaved-casepase3 (Cell Signaling Technologies, #9664), EMT kit(Cell Signaling Technologies, #9782), β-actin (Cell Signaling Technologies, #4970), Ki67 (Abcam, ab92742),AKR1C1(Abcam,ab179448), mouse IgG (Cell Signaling Technologies, #7076), and rabbit IgG (Cell Signaling Technologies, #7074). Medroxyprogesterone acetate(MPA) and ABX-1431 were obtained from Abcam and Selleck, respectively, and were both diluted in DMSO.
Ma X., Xia M., Wei L., Guo K., Sun R., Liu Y., Qiu C, & Jiang J. (2022). ABX-1431 inhibits the development of endometrial adenocarcinoma and reverses progesterone resistance by targeting MGLL. Cell Death & Disease, 13(12), 1067.
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3

Western Blot Analysis of Lung Proteins

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Lung tissue was homogenized in RIPA buffer supplemented with protease inhibitor cocktail (Roche, Indianapolis, IN). Ten micrograms of total protein were separated on SDS-PAGE gels, electroblotted onto polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories, Hercules, CA) and blocked with 5% non-fat dry milk in 0.1% Tween 20 (in PBS). Antibodies against AHR (1:1000, Enzo Life Sciences), cleaved-casepase-3 (1:1000; Cell Signaling), SOD2 (1:1000; R&D Systems), HO-1, MMP9 (1:1000; Abcam), TIMP-1 (1:1000; Abcam), tubulin (1:10,000; Sigma-Aldrich) and total actin (1:50,000, EMD Millipore) were used to assess changes in relative expression. Proteins were visualized using HRP-conjugated secondary antibodies (1:10,000) followed by enhanced chemiluminescence (ECL) and imaged using a ChemiDoc™ XRS+ System (Bio-Rad Laboratories; Hercules, CA).
Guerrina N., Traboulsi H., de Souza A.R., Bossé Y., Thatcher T.H., Robichaud A., Ding J., Li P.Z., Simon L., Pareek S., Bourbeau J., Tan W.C., Benedetti A., Obeidat M., Sin D.D., Brandsma C.A., Nickle D.C., Sime P.J., Phipps R.P., Nair P., Zago M., Hamid Q., Smith B.M., Eidelman D.H, & Baglole C.J. (2021). Aryl hydrocarbon receptor deficiency causes the development of chronic obstructive pulmonary disease through the integration of multiple pathogenic mechanisms. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(3), e21376.
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