Concanavalin A (Con A) and lipopolysaccharide (LPS) were purchased from ApexBio Technology Co., Ltd. and Sigma-Aldrich (Shanghai) Trading Co., Ltd., respectively. Mouse ELISA kits for IL-4 (20220111-20186A), IFN-γ (20220111-20140A), TNF-α (20220111-20852A), MCP-1 (20220111-20248A), IL-1 β (20220111-20174A), and IL-12 (20220111-20166A) were purchased from Shanghai Enzyme Linked Biotechnology Co., Ltd. The following flow cytometry antibodies were purchased: Brilliant Violet 605™ anti-Mouse CD45 (BioLegend), BV421 Rat anti-mouse CD25 (BD); BV510 Hamster anti-mouse CD3e (BD); mCD8-R208-APC (Sino Biological); mCD4-R711-FITC (Sino Biological); FITC anti-Mouse MHC-II (I-A/I-E) (eBioscience); BV421 Hamster anti-Mouse CD80 (BD); PE Rat anti-Mouse CD86 (BD); and BV786 Hamster ant-mouse CD11c (BD).
Bv510 hamster anti mouse cd3e
Manufactured by BD
The BV510 Hamster anti-mouse CD3e is a fluorescently labeled antibody used for the detection and analysis of mouse CD3e-positive cells in flow cytometry applications. It binds specifically to the CD3e subunit of the T-cell receptor complex, which is expressed on the surface of T-lymphocytes.
Automatically generated - may contain errors
Lab products found in correlation
Concanavalin a con a, by Apexbio (1 mentions)
Bv421 rat anti mouse cd25, by BD (1 mentions)
Pe rat anti mouse cd86, by BD (1 mentions)
Brilliant violet 605 anti mouse cd45, by BioLegend (1 mentions)
Red blood cell lysis buffer, by Thermo Fisher Scientific (1 mentions)
Apc rat anti mouse cd19, by BD (1 mentions)
Pe rat anti mouse cd11b, by BD (1 mentions)
Mouse fc block, by BD (1 mentions)
Apc cy7 rat anti mouse cd45, by BD (1 mentions)
Pe cy7 rat anti mouse cd8a, by BD (1 mentions)
Percp cy5.5 rat anti mouse cd4, by BD (1 mentions)
Triple pure high impact zirconium 1.5 mm beads, by Benchmark Scientific (1 mentions)
Pe rat anti mouse cd41, by BD (1 mentions)
Cytometric bead array mouse inflammation kit, by BD (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using bv510 hamster anti mouse cd3e
1
Murine Immune Profiling by ELISA and Flow Cytometry
2
Immunology Protocol: Characterizing Murine Leukocytes
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The following reagents were used in this study: wild-type smooth strain Escherichia coli (E. coli) (O55:B5, ATCC® 12014™), LPS from E. coli (0111:B4) and RIPA buffer with protease inhibitor cocktail were from Sigma-Aldrich, St Louis, Missouri), pyrogen-free saline (Thermo Fisher Scientific, North Ryde, New South Wales, Australia), Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD Biosciences, North Ryde, New South Wales, Australia), bovine serum albumin (BSA) (Sigma-Aldrich), alkaline phosphatase (AP) conjugated anti-mouse (Sigma-Aldrich), anti-rabbit and anti-human IgG (Sigma-Aldrich), β2GPI (Hematologic Technologies, Essex Junction, Vermont), isotype control rabbit polyclonal IgG (BD Biosciences) and red blood cell lysis buffer (eBioscience, San Diego, California). Triple-Pure High Impact Zirconium 1.5 mm beads (Benchmark Scientific, NJ) Affinity purified murine IgG2, anti-β2GPI monoclonal antibody and affinity purified polyclonal rabbit anti-β2GPI antibody were produced as previously described22 (link), 23 .
PE-rat anti-mouse CD41, APC-Cy7 rat anti-mouse CD45, BV510 hamster anti-mouse CD3e, PerCP-Cy5.5 rat anti-mouse CD4, PE-Cy7 rat anti-mouse CD8a, APC rat anti-mouse CD19, V450 rat anti-mouse LY-6G and LY-6C, PE rat anti-mouse CD11b and mouse Fc blockTM were purchased from BD Biosciences and used at 0.2 mg/mL.
PE-rat anti-mouse CD41, APC-Cy7 rat anti-mouse CD45, BV510 hamster anti-mouse CD3e, PerCP-Cy5.5 rat anti-mouse CD4, PE-Cy7 rat anti-mouse CD8a, APC rat anti-mouse CD19, V450 rat anti-mouse LY-6G and LY-6C, PE rat anti-mouse CD11b and mouse Fc blockTM were purchased from BD Biosciences and used at 0.2 mg/mL.
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