To distinguish apoptotic from normal cells, Hoechst 33342 staining (Solarbio, China) was used for the qualitative analyses of PC12 cells [43 (link)]. Briefly, after treatment, cells are incubated with Hoechst 33342 for 15 min at room temperature, rinsed three times with phosphate-buffered saline (PBS), and then captured under an inverted fluorescence microscopy (Leica Microsystems, Wetzlar, Germany).
Hoechst 33342 staining
Manufactured by Solarbio
Sourced in China
Hoechst 33342 is a fluorescent dye used for staining nuclei in living cells. It binds to DNA, emitting blue fluorescence when excited by ultraviolet light.
Automatically generated - may contain errors
3 protocols using hoechst 33342 staining
1
Hoechst 33342 Staining for Apoptosis
2
Apoptosis Detection in Paraffin Sections
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Paraffin‐embedded TGO samples were sectioned into 5‐μm slices at timed intervals, stained using a TUNEL Apoptosis Detection Kit (Vazyme Biotech, Nanjing, China) following the manufacturer's protocol, and subjected to Hoechst 33342 staining (Solarbio, Beijing, China). Unstained sections were stained with hematoxylin and eosin (H&E; Solarbio). The images were visualized using a fluorescent microscope.
3
Oxaliplatin Resistance in Colorectal Cancer
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Human colon cancer cell line SW480 and human embryonic kidney cell line 293T were purchased from ATCC. To induce OXA resistant SW480 cells, the cells were seeded onto 24 well plate before chemotherapeutics treatment. Next, SW480 cells were cultured in the presence of 0.5 mM oxaliplatin (OXA) for 24 hours followed by three days in fresh medium without a drug. This procedure was continued for six months with drug concentration increase 0.4μM per month, and the nal concentration is 2.5 mM.
Both of SW480 and SW480/OXA cells were cultured in Dulbecco Modi ed Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin solution in a cell incubator at 37 °C, 5% CO 2 , with saturated humidity.
Hoechst 33342 staining Cells were seeded onto bronectin coated 12 well plate, after treatment, 5 mg/ml Hoechst 33342 staining solution (Solarbio, Beijing, China) was added to cells and incubated for 20 mins at room temperature. Then cells were washed with PBS for three time. Images were collected by Nikon T1-SAM.
Both of SW480 and SW480/OXA cells were cultured in Dulbecco Modi ed Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin solution in a cell incubator at 37 °C, 5% CO 2 , with saturated humidity.
Hoechst 33342 staining Cells were seeded onto bronectin coated 12 well plate, after treatment, 5 mg/ml Hoechst 33342 staining solution (Solarbio, Beijing, China) was added to cells and incubated for 20 mins at room temperature. Then cells were washed with PBS for three time. Images were collected by Nikon T1-SAM.
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