G 6976

Manufactured by Santa Cruz Biotechnology

Gö6976 is a potent and selective inhibitor of protein kinase C (PKC) isoforms. It primarily targets the classical PKC isoforms α, βI, and βII. Gö6976 is commonly used as a research tool to study the role of these PKC isoforms in various cellular processes.

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2 protocols using g 6976

Purification and Analysis of Recombinant CGL

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Natural CGL was isolated as described previously^{16 (link)}. Recombinant CGL was prepared according to our previous study^{19 (link)} and further purified using Pierce High Capacity Endotoxin Removal Spin Columns (Thermo Fisher Scientific, USA). LPS (from Escherichia coli 0111:B4), N-acetylcysteine (NAC), PD98059, SP600125, SB203580, PDTC and mouse antibodies against mouse phospho-ERK1/2, phospho-JNK1/2, phospho-p38 and actin were purchased from Sigma-Aldrich (St. Louis, MO). Gö6976, Rottlerin, Wortmannin, LY294002, sc-3060, and antibodies against phospho-PKCα/δ, IL-1β, iNOS, COX-2 and IRAK2, as well as secondary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). IL-1β, IL-6, TNF-α and MCP-1 ELISA kits were purchased from R&D Systems (Minneapolis, MN). Pierce™ LAL Chromogenic Endotoxin Quantitation Kit was purchased from Thermo Scientific (Rockford, IL).

Chernikov O.V., Wong W.T., Li L.H., Chikalovets I.V., Molchanova V.I., Wu S.H., Liao J.H, & Hua K.F. (2017). A GalNAc/Gal-specific lectin from the sea mussel Crenomytilus grayanus modulates immune response in macrophages and in mice. Scientific Reports, 7, 6315.

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Ingenol Compounds Modulate HIV Latency

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The natural compound ingenol-3-dodecatrienoates extracted from Euphorbiaceae and the derivate esters Ingenol A, B and C were isolated by Kyolab Laboratories (São Paulo, Brazil). Ingenol 3,20-dibenzoate, PMA, prostatin and the PKC inhibitors Gö 6976, Gö 6983 and Ro-31–8220 were obtained from Santa Cruz Biotechnology. SAHA was obtained from SelleckChem (Houston, USA). All these compounds were diluted to specific concentrations in DMEM or RPMI, and the final concentration of solvent (DMSO) was always less than 1%. We used 20 ng/ml of TNF-α (eBiosciences) as positive control of reactivation. Levels of P-TEFb components were evaluated by immunoblotting using specific antibodies anti-CDK9 and anti-Cyc T1 (Santa Cruz Biotechnologies, cat. number 484 and 10750, respectively). Anti-tubulin (Abcam, cat. number 56676) was used to normalize proteins level.

José D.P., Bartholomeeusen K., da Cunha R.D., Abreu C.M., Glinski J., da Costa T.B., Rabay A.F., Filho L.F., Dudycz L.W., Ranga U., Peterlin B.M., Pianowski L.F., Tanuri A, & Aguiar R.S. (2014). Reactivation of latent HIV-1 by new semi-synthetic ingenol esters. Virology, 0, 328-339.

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