A Leica DMi8 Live Cell Imaging System, equipped with an OKOLab stage-top live cell incubation system, LASX software, Leica HCX PL APO 63x/1.40–0.60na oil objective, Lumencor LED light engine, CTRadvanced+ power supply, and a Leica DFC900 GT camera, was used to acquire images. Exposure times were set at 50 ms (GFP, 470 nm) and 200 ms (mCherry, 550 nm), with LED light sources at 50% power, and images acquired every 30 seconds over a 10 min time course. TIRF imaging was conducted using a 100X TIRF compatible objective and 10% laser power.
Hcx pl apo 63x 1.40 0 60na oil objective
Manufactured by Lumencor
The HCX PL APO 63x/1.40–0.60na oil objective is a high-numerical aperture objective lens designed for use in microscopy applications. It provides a magnification of 63x and a numerical aperture range of 1.40 to 0.60, allowing for high-resolution imaging and light collection. The objective is compatible with oil immersion technology.
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2 protocols using hcx pl apo 63x 1.40 0 60na oil objective
1
Live Cell Imaging of Fluorescent Proteins
2
Confocal and Widefield Microscopy of Cells
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Confocal Microscopy: Confocal images of fixed cells were collected with a Zeiss LSM 700 laser scanning microscope using ZEN Black 2012 software. Fluorescence images were colorized and overlaid using FIJI software.
Widefield Microscopy: A Leica DMi8 Live Cell Imaging System, equipped with an OKOLab stagetop live cell incubation system, LASX software, Leica HCX PL APO 63x/1.40-0.60na oil objective, Lumencor LED light engine, CTRadvanced+ power supply, and a Leica DFC900 GT camera, was used to acquire images. Exposure times were set at 200 ms (mCherry, 550 nm) and 50 ms (GFP, 480 nm), with LED light sources at 50% power, and images acquired every 30 seconds over specified time course.
Widefield Microscopy: A Leica DMi8 Live Cell Imaging System, equipped with an OKOLab stagetop live cell incubation system, LASX software, Leica HCX PL APO 63x/1.40-0.60na oil objective, Lumencor LED light engine, CTRadvanced+ power supply, and a Leica DFC900 GT camera, was used to acquire images. Exposure times were set at 200 ms (mCherry, 550 nm) and 50 ms (GFP, 480 nm), with LED light sources at 50% power, and images acquired every 30 seconds over specified time course.
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