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Pgl3 promoter

Manufactured by Genechem
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Sourced in China

The PGL3 promoter is a DNA sequence that acts as a promoter, a regulatory region that controls the transcription of a gene. It is commonly used in genetic engineering and research applications.

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Lab products found in correlation

Dual luciferase reporter assay system, by Promega (1 mentions) Dual luciferase assay, by Promega (1 mentions)

Close spelling variants of this product

PGL3 promoter vector (4 citations)

2 protocols using pgl3 promoter

1

Validating miR-9 Binding to UHRF1 3'-UTR

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The wild-type or mutant 3′-UTR of UHRF1 containing the putative miR-9 binding site were cloned into pGL3 promoter (GeneChem), individually. One microgram vectors were cotransfected into HCT116 and HT29 cells in 24-well plates, together with 100 nM miR-9 or miR-control using Lipofectamine 2000. After 48 h, luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega). Normalized luciferase activity was reported as firefly/Renilla luciferase activity. Each transfectant was assayed in triplicate.
Zhu M., Xu Y., Ge M., Gui Z, & Yan F. (2015). Regulation of UHRF1 by microRNA-9 modulates colorectal cancer cell proliferation and apoptosis. Cancer Science, 106(7), 833-839.
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2

Investigating circRELL1 and EPHB3 Interactions

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The 3′ UTR sequences of circRELL1 and EPHB3 were cloned into a pGL3 promoter (Genechem, Shanghai, China). The reporter plasmid, miR-637 mimics and negative control were transfected into cells using lipofectamine 3000. The Dual-Luciferase Assay (Promega, Madison, WI, USA) was utilized to investigate the luciferase activities in the treated Cells.
Sang H., Zhang W., Peng L., Wei S., Zhu X., Huang K., Yang J., Chen M., Dang Y, & Zhang G. (2022). Exosomal circRELL1 serves as a miR-637 sponge to modulate gastric cancer progression via regulating autophagy activation. Cell Death & Disease, 13(1), 56.
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