Improm 2 reverse transcription system a3800

Manufactured by Promega

Sourced in United States

The ImProm-II™ Reverse Transcription System A3800 is a laboratory equipment product designed for the reverse transcription of RNA into complementary DNA (cDNA). It provides the necessary components for the process of reverse transcription, including the ImProm-II™ Reverse Transcriptase enzyme, reaction buffer, and necessary reagents.

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Lab products found in correlation

Iq5 real time pcr, by Bio-Rad (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Reverse Transcription System (942 citations) ImProm-II Reverse Transcription System (737 citations) ImProm-II (157 citations) ImProm Reverse Transcription System (8 citations) ImProm-II™ reverse (4 citations) Reverse Transcription II system (4 citations) A3800 (3 citations) ImProm-II Reverse Transcription (2 citations)

2 protocols using improm 2 reverse transcription system a3800

Pulmonary Cytokine Expression Analysis

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Pulmonary cytokines expression was assessed as described previously [18 (link)]. qRT-PCR TRIzol reagent (ThermoFisher Scientific, Waltham, MA, USA) was used to isolate RNA from mouse lungs. Then, reverse transcription was performed using ImProm-II™ Reverse Transcription System A3800 (Promega, Madison, WI, USA), following the manufacturer’s instructions. Detection was performed using the Bio-Rad iQ5 real-time PCR. Results were analyzed as 2^−ΔΔCt, and β-actin was used for normalization. The following primers were used (forward and reverse):
IL-6 mouse: F:TAGTCCTTCCTACCCCAATTTCC;
R: TTGGTCCTTAGCCACTCCTTC
PrimerBank ID: 13624311a1
IL-4 mouse: F: GGTCTCAACCCCCAGCTAGT;
R: GCCGATGATCTCTCTCAAGTGAT
PrimerBank ID: 10946584a1
IFN-γ mouse: F: ATGAACGCTACACACTGCATC;
R: CCATCCTTTTGCCAGTTCCTC
PrimerBank ID: 33468859a1
IL-17A mouse: F: TTTAACTCCCTTGGCGCAAAA;
R: CTTTCCCTCCGCATTGACAC
PrimerBank ID: 6754324a1
β-actin mouse: F: GTGACGTTGACATCCGTAAAGA;
R: GCCGGACTCATCGTACTCC
PrimerBank ID: 145966868c1

Vicente J., Benedetti M., Martelliti P., Vázquez L., Gentilini M.V., Peñaranda Figueredo F.A., Nabaes Jodar M.S., Viegas M., Barquero A.A, & Bueno C.A. (2023). The Flavonoid Cyanidin Shows Immunomodulatory and Broad-Spectrum Antiviral Properties, Including SARS-CoV-2. Viruses, 15(4), 989.

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Isolation and Quantification of Mouse Lung RNA

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RNA from mouse lung tissue was isolated using TRIzol reagent (Life Technologies) and reverse transcribed with ImProm-II™ Reverse Transcription System A3800 (Promega), according to the manufacturer's instructions. Quantitative PCR was performed on the Bio-Rad iQ5 real-time PCR system using FastStart SYBR green Master Mix reagent (Roche). β-actin was used as an internal control for normalization. The data were

Salinas F.M., Vázquez L., Gentilini M.V., O Donohoe A., Regueira E., Nabaes Jodar M.S., Viegas M., Michelini F.M., Hermida G., Alché L.E, & Bueno C.A. (2019). Aesculus hippocastanum L. seed extract shows virucidal and antiviral activities against respiratory syncytial virus (RSV) and reduces lung inflammation in vivo. Antiviral research, 164.

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