Unicel dxh 800 cellular analysis system

Venous blood samples were obtained and administered to Vacutainer™ pneumatic tubes (Becton Dickinson, Meylan Cedex, France). EDTA blood was used for full blood count. Hemogram was determined with Beckman Coulter UniCel®DxH800 Cellular Analysis System (Beckman Coulter, Miami, Florida, USA). Citrated platelet poor plasma was prepared by double centrifugation at 2500 × g for 15 min at room temperature and stored in 1.0 mL aliquots at − 80 °C until testing fibrinogen, prothrombin fragment F1 + F2 and D-dimer. Coagulation tests were carried out on a Behring coagulation system (BCS) ™ analyzer (Dade Behring, Marburg, Germany). Determination of F IIa (trombin) inhibition and concentration level of prothrombin fragment F1 + 2 were carried out according to the manufacturer‘s instructions using the MRX Microplate Reader™ microphotometer (Dynatech Laboratories, Chantilly, USA). Fibrinogen was determined using the Fibrinogen reagent kit (product supplied By Technoclone, Austria). Prothrombin fragment F1 + 2 was determined using the Enzygnost® F 1 + 2 micro kit (product supplied by Dade Behring, Marburg, Germany). D-dimer was determined using the ELISA D dimer kit (product supplied by Boehring-Mannheim, Germany). Acute phase proteins (CRP, Oroso, Trf) were determined using the Nephelometer BNII (Dade Behring, Marburg, Germany).