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Zr viral rna extraction kit

Manufactured by Zymo Research
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The ZR Viral RNA extraction kit is a laboratory equipment used to extract viral RNA from various sample types. It utilizes a silica-based membrane technology to efficiently capture and purify viral RNA, which can then be used for downstream applications such as RT-qPCR or RNA sequencing.

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Lab products found in correlation

Taqman universal master mix, by Thermo Fisher Scientific (2 mentions) Rneasy kit, by Qiagen (2 mentions) Iscript select cdna synthesis kit, by Bio-Rad (2 mentions) Rlt solution, by Qiagen (2 mentions) 7300 rt pcr machine, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

RNA extraction kit (66 citations) ZR Viral RNA (3 citations) ZR viral kit (2 citations)

2 protocols using zr viral rna extraction kit

1

Quantifying ZIKV RNA in Maternal-Fetal Tissues

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Viral RNA load was assessed in tissues from the dam, fetus, and placenta using a ZIKV-specific RT-qPCR assay. Fetal and maternal organs were immersed in RNA-later immediately upon harvest and were then weighed and homogenized in RLT solution (Qiagen) using a bead-beater apparatus (Precellys). RNA was extracted from tissues using the RNeasy kit (Qiagen) and from sera using the ZR Viral RNA extraction kit (Zymo Research). From tissues, 400 ng of total RNA was used to generate cDNA using the iScript select cDNA synthesis kit (Bio-Rad) according to manufacturer’s protocols for gene-specific primers. Viral RNA was quantified using the Taqman Universal Master Mix (Applied Biosystems) and an Applied Biosystems 7300 RT-PCR machine with primers that correspond to residues conserved in both the FSS13025 and Brazil Fortaleza genome (GenBank numbers KU955593.10, KX811222.1).49 (link) To adhere to stringent guidelines, Ct (cycle threshold) values >38 were deemed as not reliably detected and were not reported. Copy number sensitivity, as determined using a standard curve from diluted known quantities of ZIKV genome, was 25 copies/qPCR reaction.
Adams Waldorf K.M., Nelson B.R., Stencel-Baerenwald J.E., Studholme C., Kapur R.P., Armistead B., Walker C.L., Merillat S., Vornhagen J., Tisoncik-Go J., Baldessari A., Coleman M., Dighe M.K., Shaw D.W., Roby J.A., Santana-Ufret V., Boldenow E., Li J., Gao X., Davis M.A., Swanstrom J.A., Jensen K., Widman D.G., Baric R.S., Medwid J.T., Hanley K.A., Ogle J., Gough G.M., Lee W., English C., Durning W.M., Thiel J., Gatenby C., Dewey E.C., Fairgrieve M.R., Hodge R.D., Grant R.F., Kuller L., Dobyns W.B., Hevner R.F., Gale M J.r, & Rajagopal L. (2018). Congenital Zika Virus Infection as a Silent Pathology With Loss of Neurogenic Output in the Fetal Brain. Nature medicine, 24(3), 368-374.
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2

Quantifying ZIKV RNA in Maternal-Fetal Tissues

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Viral RNA load was assessed in tissues from the dam, fetus, and placenta using a ZIKV-specific RT-qPCR assay. Fetal and maternal organs were immersed in RNA-later immediately upon harvest and were then weighed and homogenized in RLT solution (Qiagen) using a bead-beater apparatus (Precellys). RNA was extracted from tissues using the RNeasy kit (Qiagen) and from sera using the ZR Viral RNA extraction kit (Zymo Research). From tissues, 400 ng of total RNA was used to generate cDNA using the iScript select cDNA synthesis kit (Bio-Rad) according to manufacturer’s protocols for gene-specific primers. Viral RNA was quantified using the Taqman Universal Master Mix (Applied Biosystems) and an Applied Biosystems 7300 RT-PCR machine with primers that correspond to residues conserved in both the FSS13025 and Brazil Fortaleza genome (GenBank numbers KU955593.10, KX811222.1).49 (link) To adhere to stringent guidelines, Ct (cycle threshold) values >38 were deemed as not reliably detected and were not reported. Copy number sensitivity, as determined using a standard curve from diluted known quantities of ZIKV genome, was 25 copies/qPCR reaction.
Adams Waldorf K.M., Nelson B.R., Stencel-Baerenwald J.E., Studholme C., Kapur R.P., Armistead B., Walker C.L., Merillat S., Vornhagen J., Tisoncik-Go J., Baldessari A., Coleman M., Dighe M.K., Shaw D.W., Roby J.A., Santana-Ufret V., Boldenow E., Li J., Gao X., Davis M.A., Swanstrom J.A., Jensen K., Widman D.G., Baric R.S., Medwid J.T., Hanley K.A., Ogle J., Gough G.M., Lee W., English C., Durning W.M., Thiel J., Gatenby C., Dewey E.C., Fairgrieve M.R., Hodge R.D., Grant R.F., Kuller L., Dobyns W.B., Hevner R.F., Gale M J.r, & Rajagopal L. (2018). Congenital Zika Virus Infection as a Silent Pathology With Loss of Neurogenic Output in the Fetal Brain. Nature medicine, 24(3), 368-374.
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