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Ezna lysis buffer total rna kit 1

Manufactured by Omega Bio-Tek
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Sourced in Italy

EZNA lysis buffer is a component of the Total RNA Kit I from Omega Bio-Tek. It is designed to facilitate the lysis and homogenization of various biological samples, enabling the efficient extraction and purification of total RNA.

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Lab products found in correlation

Itaq universal sybr green one step kit, by Bio-Rad (2 mentions) Dnase 1, by Omega Bio-Tek (1 mentions)

Close spelling variants of this product

RNA lysis buffer (2 citations)

2 protocols using ezna lysis buffer total rna kit 1

1

Quantification of Gene Expression in LMMP Samples

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Total RNA was extracted from LMMP samples using the EZNA lysis buffer (Total RNA Kit I, Omega Bio-tek, Italy). Contaminating DNA was removed through DNase I treatment (Omega Bio-tek). Gene expression was assessed using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad Laboratories; Segrate, Italy). The expression of the targeted mRNA was normalized to 18S ribosomal RNA (Rn18S) and plotted as mean fold expression. Oligonucleotides and annealing temperatures used for qRT-PCR are listed in Table 1.
Bernabè G., Shalata M.E., Zatta V., Bellato M., Porzionato A., Castagliuolo I, & Brun P. (2023). Antibiotic Treatment Induces Long-Lasting Effects on Gut Microbiota and the Enteric Nervous System in Mice. Antibiotics, 12(6), 1000.
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2

RNA Extraction and qRT-PCR Analysis

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Total RNA was extracted from LMMP using the EZNA lysis buffer (Total RNA Kit I, Omega bio-tek, Italy) and the Retsch MM300 TissueLyser Lab Vibration Mill Mixer (QIAGEN S.r.l., Milan, Italy) (Brun et al., 2013 (link)). Contaminating DNA was removed by DNase I treatment (Omega bio-tek). Gene expression was assessed using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad Laboratories; Segrate, Italy); oligonucleotides are listed in Table 1. The expression of the targeted mRNA was normalized to 18S ribosomal RNA (Rn18S) and plotted as mean fold expression.
Brun P., Conti J., Zatta V., Russo V., Scarpa M., Kotsafti A., Porzionato A., De Caro R., Scarpa M., Fassan M., Calistri A, & Castagliuolo I. (2021). Persistent Herpes Simplex Virus Type 1 Infection of Enteric Neurons Triggers CD8+ T Cell Response and Gastrointestinal Neuromuscular Dysfunction. Frontiers in Cellular and Infection Microbiology, 11, 615350.
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