Quercetin dehydrate, α-tocopherol, l -α-lysophosphatidylcholine (lysoPC) and rabbit polyclonal antibody to CAV-1 were purchased from Sigma-Aldrich (St. Louis, MO). Human LDL and human oxidized-LDL (low-thiobarbituric acid reactive) were obtained from Biomedical Technologies (Stoughton, MA). Q3GA was obtained from Extrasynthese (Genay, France). 13-Hydroperoxyoctadecadienoic acid (13-HPODE) was obtained from Larodan Fine Chemicals AB (Malmo, Sweden). 13(S)-Hydroxyoctadecadienoic acid (13-HODE) was obtained from Cayman Chemical (Ann Arbor, MI). 4-Hydroxynonenal (4-HNE) was obtained from Percipio Biosciences (Burlingame, CA). Rabbit polyclonal antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and anti-phospho-CAV-1 antibody were obtained from Cell Signaling Technology (Beverly, MA). Secondary antibody, polyclonal goat anti-rabbit immunoglobulin/horseradish peroxidase was obtained from Dako (Glostrup, Denmark).
Human ldl
Manufactured by Biomedical Technologies
Human LDL is a laboratory product that measures the concentration of low-density lipoprotein (LDL) in human blood samples. LDL is a type of cholesterol that is often referred to as 'bad' cholesterol due to its potential to contribute to the buildup of plaque in the arteries.
Automatically generated - may contain errors
Lab products found in correlation
Quercetin dehydrate, by Merck Group (1 mentions)
α tocopherol, by Merck Group (1 mentions)
Modified lowry protein assay kit, by Thermo Fisher Scientific (1 mentions)
Alkaline phosphatase conjugated anti rabbit igg, by Merck Group (1 mentions)
Cholesterol e test wako, by Fujifilm (1 mentions)
Oleic acid, by Merck Group (1 mentions)
4 protocols using human ldl
1
Oxidative Stress Pathway Modulation
2
Quantification of Synthetic Lipoproteins
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Synthetic AIPs were purchased from Biopetide Co., Inc. (San Diego, CA). Human LDL was purchased from Biomedical Technologies, Inc., (Stoughton MA). Superose 6 10/300 prepacked columns were obtained from GE Healthcare Life Sciences. Other reagents were obtained as follows: rabbit anti-mouse apoB polyclonal antibody (LS-C20729, LifeSpan Biosciences); alkaline phosphatase conjugated anti-rabbit IgG (Sigma-Aldrich); nitro-blue tetrazolium and 5-bromo-4-chloro-3′-indolyphosphate (NBT/BCIP, Pierce), Modified Lowry Protein Assay Kit (Thermo Scientific); 3–8% Tris-Acetate protein gel (NuPAGE, Novex; Life Technologies); 4-aminopyrazole-(3,4-D)pyrimidine (4APP), (Sigma-Aldrich).
3
Lipoprotein Preparation and Cholesterol Assay
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Squalestatin and oleic acid were obtained from Sigma (St. Louis, MO). Human LDL (density 1.019–1.063 g/mL) was purchased from Biomedical Technologies Inc. (Stoughton, MA). Lipoprotein-deficient serum (LPDS) was prepared by ultracentrifugation of FBS after its density was increased to 1.215 g/mL with potassium bromide [4 (link)]. The cholesterol concentration in the LPDS was estimated to be under the minimum level detectable by a commercial detection kit (Cholesterol E-test Wako, Wako Pure Chemical Industries).
4
Quantification of Modified LDL Antibodies
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Serum from all patients was screened for the presence of anti-MDA-LDL and MAA-LDL IgG antibodies. Human LDL (Biomedical Technologies, Inc, Ward Hill, MA) was modified with MDA as previously described [33] (link). Briefly, Human LDL was reacted with 0.2 M MDA for 3 hours at 37 degrees. Human LDL was MAA modified by reacting 2 mM MDA with 1 mM acetaldehyde as previously described [25] (link). ELISA plates were coated with MDA-LDL, MAA-LDL, and LDL alone and serum tested for reactivity as describe above.
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