Staphylococcus epidermidis

Endophytic Streptomyces sp. SP5 (GenBank accession number MW564023) isolated from Citrus jambhiri leaves was used in the current study^{14 (link)}. The streptomycete was maintained on starch casein nitrate agar (SCNA) plates, and spores of isolate were preserved in 20% glycerol at − 20 °C as stock for future use. Different test pathogenic bacteria viz. Bacillus subtilis (MTCC 619), Klebsiella pneumoniae subsp. pneumoniae (MTCC 109), E. coli (MTCC 1885), Staphylococcus epidermidis (MTCC 435), Salmonella typhi (MTCC 733), Alternaria brassicicola (MTCC2102), Colletotrichum acutatum (MTCC1037), Alternaria solani (MTCC2101), Cladosporium herbarum (MTCC351) and Fusarium oxysporum (MTCC284) were obtained from the Microbial Type Culture Collection (MTCC) and Gene Bank, CSIR-Institute of Microbial Technology (IMTECH), Chandigarh, India. Fusarium solani (NFCCI 91) was obtained from the NFCCI (National Fungal Culture Collection of India), Pune, and Alternaria alternata (accession number GU004283) and Fusarium moniliforme were isolated in the lab (PAU). VRE (resistant to methicillin, vancomycin, imipenem, and clindamycin) and MRSA (resistant to methicillin, teicoplanin, imipenem, and clindamycin) were collected from local hospitals. The bacterial and fungal cultures were maintained at 4 °C on nutrient agar and potato dextrose agar slants, respectively.

All the test microbial pathogens such as Staphylococcus aureus (MTCC 7443), Bacillus subtilis (MTCC 121), Bacillus cereus (MTCC 430), Staphylococcus epidermidis (MTCC 435), Escherichia coli (MTCC 7410), Klebsiella pneumonia (MTCC 7407), Salmonella typhi (MTCC 733), Shigella flexneri (MTCC 1457), Vibrio parahaemolyticus ((MTCC 451), Xanthomonas campestris (MTCC 7908), and Candida albicans (MTCC 183) were procured from Microbial Type Culture Collection and Gene Bank (MTCC; Chandigarh, India).