Mouse rip1 monoclonal antibody

Tissue sections were treated as described above. After treatment with 0.1%Triton X-100, the sections were blocked with 10% goat serum and incubated overnight at 4°C with a mouse RIP1 monoclonal antibody (Abcam, Cambridge, MA, USA; 1:100 dilution) and a rabbit RIP3 polyclonal antibody (Abcam, Cambridge, MA, USA; 1:100 dilution), followed by incubation with Alexa Fluor 594-conjugatedgoat anti-mouse IgG and Alexa Fluor 488-conjugatedgoat anti-rabbit IgG (Beyotime, Nantong, Jiangsu, China). After three washes with 0.1 MPBS, the sections were counterstained with DAPI. Finally, the numbers of total cells and RIP1/-RIP3-positive cells were determined using a laser scanning confocal microscope (LEICA TCS SP2, Wetzlar, Germany).

Tissue sections were treated as described above. After treatment with 0.1% Triton X-100, the sections were blocked with 10% goat serum and incubated over night at 4 °C with a mouse RIP1 monoclonal antibody (Abcam, Cambridge, MA, USA; 1:100 dilution) and a rabbit RIP3 polyclonal antibody (Abcam, Cambridge, MA, USA; 1:100 dilution), followed by incubation with Dylight 594 AffiniPure goat anti-mouse IgG and Dylight 488 AffiniPure goat anti-rabbit IgG (EarthOx, San Francisco, CA, USA). After three washes with 0.1 MPBS, the sections were counterstained with DAPI. Finally, the numbers of total cells and RIP1/-RIP3-positive cells were determined using a laser scanning confocal microscope (LEICA TCS SP2, Wetzlar, Germany).