The Cell Death Detection ELISAPLUS photometric enzyme immunoassay kit (Roche Diagnostics, Indianapolis, IN, USA) was used to analyze the apoptotic and necrotic cell death quantitatively after 24 hr. The kit utilizes two monoclonal antibodies directed against DNA and histones, respectively, to allow the specific determination of mono- and oligo-nucleosomes in the cytoplasmatic fraction (apoptosis) or in the supernatant (necrosis) of cells.
Cell death detection elisaplus photometric enzyme immunoassay kit
Manufactured by Roche
Sourced in Germany
The Cell Death Detection ELISAPLUS Photometric Enzyme Immunoassay Kit is a laboratory equipment product manufactured by Roche. The kit is designed to detect and quantify cell death, specifically the presence of histone-complexed DNA fragments, in a photometric enzyme-linked immunosorbent assay (ELISA) format.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using cell death detection elisaplus photometric enzyme immunoassay kit
1
Quantitative Cell Death Analysis
2
Apoptosis Quantification via ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis was also verified by using Cell Death Detection ELISAPLUS Photometric Enzyme Immunoassay Kit (Roche Diagnostics GmbH, Mannheim, Germany). In brief, 1 × 104 cells were harvested and lyzed in 200 μl of lysis buffer provided by the kit for 30 min at room temperature followed by centrifugation at 200 × g for 10 min. 20 μl of supernatant was then added to ELISA microplate for quantification of cytoplasmic histone-associated DNA fragments according to the manufacturer's instructions.
3
Apoptosis Detection by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis was also analyzed using Cell Death Detection ELISAPLUS Photometric Enzyme Immunoassay Kit (Roche Diagnostics GmbH, Mannheim, Germany). In brief, cells were harvested and lyzed in lysis buffer provided by the kit after treatment. Cytoplasmic histone-associated DNA fragments after induced apoptosis were finally quantified according to the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!