Mkn45

The human cancer cell lines including AGS, HGC27, HeLa, Panc-1, HCT116, A549, and U2OS were purchased from the Cell Resource Center, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). MKN45 was purchased from the Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Beijing, China). Hucct1 were obtained from the Japanese Collection of Research Bioresources (JCRB) (Tokyo, Japan). The cell lines were originally authenticated in China Center for Type Culture Collection (CCTCC) by Short Tandem Repeat (STR) profiling and passaged less than 6 months in the lab. These cells are routinely maintained in the laboratory of our group.The acid cell culture medium (pH 6.5) was obtained by the addition of 1 M HCl solution as previously reported^{23 (link),24 (link)}. The pH was estimated by the use of a FE20 FiveEasy Plus pH Meter (METTLER TOLEDO Instruments (China), Shanghai, China).

GES-1 gastric epithelial cells and the GC cell lines HGC-27, AGS, MKN45, and NCI-N87 were purchased from the Cell Resource Center, Peking Union Medical CollegePMUC (Beijing, China). All cells were maintained in Dulbecco’s modified Eagle’s medium (RPMI-1640; Gibco, USA) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin and streptomycin (Gibco). All cells were cultured in a 5% CO² humidified atmosphere at 37°C (Baust et al., 2017 (link)).

Gastric cancer cell lines MKN45 and SGC7901, human embryonic kidney cell line HEK-293T and human gastric mucosa cell line GES-1 were purchased from the Shanghai Institutes for Biological Sciences Cell Resource Center, Shanghai, China. MKN45 cells and HEK-293T cells were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (Life Technologies Corporation, Paisley, UK). SGC7901 and GES-1 cells were cultured in RPMI-1640 (Sigma-Aldrich, St Louis, MO, USA) containing 10% fetal bovine serum. All cells were incubated in a humidified incubator at 37 °C with 5% CO₂.

Human gastric cancer cell lines (HGC-27, MKN-45, MGC-803, SGC-7901) and human gastric mucosal cells (GES-1) were purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, China. Cell lines were maintained in RPMI-1640 medium containing 10% fetal bovine serum (FBS), penicillin (100 U/mL), and streptomycin (100 mg/mL) (Invitrogen, USA) at 37 ºC with 5% CO₂.