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Cd20 pacific blue

Manufactured by BioLegend
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Sourced in United Kingdom

The CD20 Pacific Blue is a laboratory reagent used for the identification and analysis of cells expressing the CD20 antigen. It is a fluorochrome-conjugated monoclonal antibody that binds specifically to the CD20 cell surface marker, which is expressed on B lymphocytes. This product is intended for research use only.

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Lab products found in correlation

Cd3 percp, by BioLegend (1 mentions) Cd19 apc cy7, by BioLegend (1 mentions) Cd8 apc cy7, by BioLegend (1 mentions) Cd161 apc, by BioLegend (1 mentions) Gallios cytometer, by Beckman Coulter (1 mentions) Cd25 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd4 pacific blue, by Thermo Fisher Scientific (1 mentions) Cd161 percpcy5, by BioLegend (1 mentions) Kaluza flow cytometry software, by Beckman Coulter (1 mentions) Pancol, by PAN Biotech (1 mentions)

Close spelling variants of this product

Anti-CD20 Pacific Blue (2 citations) CD20 Pacific Blue (2H7; (2 citations)

2 protocols using cd20 pacific blue

1

Multiparameter Flow Cytometry of PBMCs

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PBMC were stained with the following antibodies: TCRC Pacific Blue, CD4 V500, V follow (Biolegend, UK), MR1 APC (26.5) (Biolegend, UK), CD3 PerCP, CD161 APC, CD8 APC-Cy7, CD19 APC-Cy7 and CD20 Pacific Blue. All antibodies were from BD biosciences unless specified. The samples were stained for 15 minutes at room temperature, processed and then analyzed on a FACS Canto II machine with FACS Diva software evaluation.
Gao Y., Rae W., Ramakrishnan K.A., Barcenas-Morales G., Döffinger R., Eren E., Faust S.N., Ottensmeier C.H, & Williams A.P. (2016). Mucosal-Associated Invariant T (MAIT) Cells Are Impaired in Th17 Associated Primary and Secondary Immunodeficiencies. PLoS ONE, 11(5), e0155059.
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2

Multiparameter flow cytometry analysis of lymphocyte subsets after allo-SCT

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T-cell (CD3/CD4), NK-cell, and B-cell absolute numbers were analyzed by our routine central laboratory using flow cytometry and the TruCount system (Becton & Dickinson, www.bd.com). For analysis of T-regulatory cells and CD3+ CD161+ NK/T cells, whole blood (30 mL) was collected in EDTA tubes at day+30, +90, and +150 after allo-SCT. Peripheral blood mononuclear cells were isolated by density-gradient centrifugation (Pancol®, www.pan-biotech.com). Cells were stained with the following fluorescence-labeled antibodies: CD20 Pacific Blue and CD161 PerCP-Cy5.5 (www.biolegend.com), CD4 Pacific Blue, CD25 PE-Cy7, CD127 AlexaF700, CD3 APC-H7 (www.bd.com), CD19 Alexa780 APC (www.eBioscience.com), CD45 Pacific Orange (www.lifetechnologies.com).
Flow cytometry was performed using a Gallios® cytometer (Beckman Coulter, www.beckmancoulter.com). Data were analyzed by Kaluza® flow cytometry software (Beckman Coulter).
Holtick U., Chemnitz J.M., Shimabukuro-Vornhagen A., Theurich S., Chakupurakal G., Krause A., Fiedler A., Luznik L., Hellmich M., Wolf D., Hallek M., von Bergwelt-Baildon M, & Scheid C. (2015). OCTET-CY: a phase II study to investigate the efficacy of post-transplant cyclophosphamide as sole graft-versus-host prophylaxis after allogeneic peripheral blood stem cell transplantation. European journal of haematology, 96(1), 27-35.
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