Alpha-Minimal Essential Medium (α-MEM), Dulbecco's modified Eagle's medium F12, and Earle's Balanced Salt Solution cell culture media were obtained from Invitrogen. Fetal calf serum was purchased from Internegocios S.A. Kanamycin (50 μg/ml), chloramphenicol (20 μg/ml), and ampicillin (100 μg/ml); MTT, firefly luciferase, suramin, PPADS, 8-phenyl theophylline, NF110, HK, apyrase, ATP, ADP, AMP, UTP, and suramin were purchased from Sigma-Aldrich. LIVE/DEAD BacLight Bacterial Viability kit and 4',6-diamidino-2-phenylindole were purchased from Molecular Probes.
Nf110
Manufactured by Merck Group
Sourced in United States
The NF110 is a laboratory filtration device designed for small-scale filtration processes. It is a compact and portable unit that utilizes a membrane filtration system to separate components within a liquid sample. The NF110 is suitable for a variety of laboratory applications that require efficient liquid filtration.
Automatically generated - may contain errors
Lab products found in correlation
Suramin, by Merck Group (2 mentions)
Apyrase, by Merck Group (2 mentions)
Ppads, by Merck Group (2 mentions)
Firefly luciferase, by Merck Group (1 mentions)
Chloramphenicol, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium f12, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Alpha minimal essential medium α mem, by Thermo Fisher Scientific (1 mentions)
Earle s balanced salt solution, by Thermo Fisher Scientific (1 mentions)
Adenosine diphosphate (adp), by Merck Group (1 mentions)
Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Ampicillin, by Merck Group (1 mentions)
Carbenoxolone, by Merck Group (1 mentions)
Imidazole, by Merck Group (1 mentions)
Probenecid, by Merck Group (1 mentions)
Mastoparan 7, by Merck Group (1 mentions)
Pom 1, by Merck Group (1 mentions)
Mrs2211, by Merck Group (1 mentions)
2 protocols using nf110
1
Cell Culture Media and Reagents
2
Luciferase Assay for P2 Receptors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All reagents in this study were of analytical grade. Poly-D-lysine, mastoparan7, bovine serum albumin, carbenoxolone, probenecid, firefly luciferase (EC 1.13.12.7), choline chloride, suramin, PPADS, NF110, imidazole, sucrose, Hepes, apyrase, POM1, 2-MeSADP, MRS 2211 and HIBA were purchased from Sigma-Aldrich (St. Louis, MO, USA). BCECF-AM and D-luciferin were purchased from Invitrogen/Molecular Probes Inc. (Eugene, OR, USA).22NaCl was purchased from New England Nuclear (MA, USA).
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