D3h2ln

HEK-293t, SW480, SW620, MCF10A and MDA-MB-231 were purchased from ATCC collection (Middlesex, UK). Human Fibroblasts correspond to early passage fibroblasts isolated from neonatal human foreskin as described previously (Baus, 2003 (link); Dazard, 2000 ). MDA-MB-231-D3H2LN was a Bioware Cell line purchased from Caliper LifeScience (Villepinte, France). Cells were regularly checked for mycoplasma contamination by using Mycoalert Detection Kit (#LT07-318) from Lonza (Levallois, France).
HEK-293t, SW480, SW620, MDA-MB-231 and D3H2LN were grown in DMEM supplemented with 10% FBS, 1 mM sodium pyruvate and antibiotics/glutamine (Invitrogen, Thermo Fisher, Illkirch, France). MCF10A cells were cultured in DMEM/F12 with 5% horse serum (Invitrogen, 1 mM sodium pyruvate, EGF (20 ng/ml, PEPROTECH, Neuilly-Sur-Seine, France), Hydrocortizone (500 ng/ml), cholera toxin (100 ng/ml), Insulin (10 μg/ml) (Sigma Aldrich, Saint-Quentin Fallavier, France) and antibiotics/glutamine (Invitrogen).
Human Fibroblasts were grown in MEM supplemented with 10% FCS, antibiotics/glutamine (Invitrogen).

The human MDA-MB-231 breast adenocarcinoma (Cat# HTB-26) and MDA-MB-157 breast medullary carcinoma (Cat# HTB-24) cell lines were purchased from the American Type Culture Collection (ATCC). The MDA-MB-231-Luc-D3H2LN Bioware (D3H2LN) cell line was purchased from PerkinElmer (Cat# 119369). Mouse 4T1 cells (Cat# CRL-2539) were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic solution (Invitrogen). D3H2LN, MDA-MB-231, and MDA-MB-157 cell lines were maintained in DMEM supplemented with 10% FBS, 1% GlutaMAX (Invitrogen), 10mM HEPES, 1mM sodium pyruvate, non-essential amino acids and 1% Antibiotic-Antimycotic solution.