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Ab7500 fast cycler

Manufactured by Thermo Fisher Scientific
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The AB7500 FAST cycler is a real-time PCR instrument designed for fast and efficient nucleic acid analysis. It features a compact design and provides accurate temperature control for reliable PCR performance.

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Lab products found in correlation

Micrornaeasy kit, by Qiagen (2 mentions) Quantitect kit, by Qiagen (2 mentions)

2 protocols using ab7500 fast cycler

1

qPCR Analysis of Mouse Kmo Gene

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We made cDNA using a Quantitect kit applied to RNA extracts of tissue made using microRNAEasy kits (Qiagen). We used inventoried TaqMan assays for mouse Kmo in a standard TaqMan FAST Assay with amplification detected on an AB7500 FAST cycler (Applied Biosystems). We performed all assays as duplex reactions with an endogenous 18S RNA internal control.
Mole D.J., Webster S.P., Uings I., Zheng X., Binnie M., Wilson K., Hutchinson J.P., Mirguet O., Walker A., Beaufils B., Ancellin N., Trottet L., Bénéton V., Mowat C.G., Wilkinson M., Rowland P., Haslam C., McBride A., Homer N.Z., Baily J.E., Sharp M.G., Garden O.J., Hughes J., Howie S.E., Holmes D.S., Liddle J, & Iredale J.P. (2016). Kynurenine–3–monooxygenase inhibition prevents multiple organ failure in rodent models of acute pancreatitis. Nature medicine, 22(2), 202-209.
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2

qPCR Analysis of Mouse Kmo Gene

Check if the same lab product or an alternative is used in the 5 most similar protocols
We made cDNA using a Quantitect kit applied to RNA extracts of tissue made using microRNAEasy kits (Qiagen). We used inventoried TaqMan assays for mouse Kmo in a standard TaqMan FAST Assay with amplification detected on an AB7500 FAST cycler (Applied Biosystems). We performed all assays as duplex reactions with an endogenous 18S RNA internal control.
Mole D.J., Webster S.P., Uings I., Zheng X., Binnie M., Wilson K., Hutchinson J.P., Mirguet O., Walker A., Beaufils B., Ancellin N., Trottet L., Bénéton V., Mowat C.G., Wilkinson M., Rowland P., Haslam C., McBride A., Homer N.Z., Baily J.E., Sharp M.G., Garden O.J., Hughes J., Howie S.E., Holmes D.S., Liddle J, & Iredale J.P. (2016). Kynurenine–3–monooxygenase inhibition prevents multiple organ failure in rodent models of acute pancreatitis. Nature medicine, 22(2), 202-209.
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