C18 solid phase extraction (SPE) columns were purchased from Waters (Sep-Pak, 10g-35cc and 500mg-6cc versions). The SPE columns were attached to a vacuum manifold (Vac Elut 20, Agilent) and pressure was maintained at 2–5psi. The column was washed with two column-volumes of methanol, then two volumes of water. Samples were typically diluted to 20% or less methanol content and acidified to pH 3.5 and loaded onto the column at a drop rate of 1–2 drops/sec. The column was washed with two volumes of water and one volume of 50% methanol. The compounds were then eluted with one volume of 100% methanol into glass test tubes. The solution was dried down using a Thermo Reacti-Vap apparatus under compressed air and the residue was dissolved in a small amount of 100% methanol.
Reacti vap
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Reacti-Vap is a laboratory evaporator designed to concentrate and dry samples efficiently. It utilizes a heating block and a gentle flow of nitrogen or air to gently evaporate solvents from samples, preserving their integrity. The Reacti-Vap provides a controlled, consistent environment for sample preparation and processing.
Automatically generated - may contain errors
3 protocols using reacti vap
1
C18 SPE for Sample Purification
2
Quantification of NVP Plasma Levels
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The NVP plasma concentrations were quantified using a tandem quadrupole mass spectrometer (LC/MS/MS) designed for ultra-high performance: Xevo TQ-S (Waters Corporation, Massachusetts) as described by Reddy et al[17 ] modified in our previous publication.[15 (link),16 (link)] Plasma samples were extracted using Bond Elut C18 cartridges, according to the manufacturer’s instructions (Agilent Technologies, California). The eluents were completely evaporated using Thermo Scientific Reacti-Vap evaporators (Thermo Fisher Scientific Inc., California) at 37°C for 30 minutes. This was then reconstituted using 100 µL of equal parts 1:1 acetonitrile and water, vortexed briefly, transferred into 50 mL capped vials, and placed into Xevo TQ-S (Waters Corporation) for quantification. Approximately 1 µL of the sample was injected automatically into the LC/MS/MS instrument and quantified within 5 minutes. NVP plasma concentrations were categorized as <3400 ng/mL (below the therapeutic range), 3400 to 6000 ng/mL (therapeutic range), or >6000 ng/mL (above the therapeutic range).[18 (link),19 (link)]
3
C18 SPE for Sample Purification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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C18 solid phase extraction (SPE) columns were purchased from Waters (Sep-Pak, 10g-35cc and 500mg-6cc versions). The SPE columns were attached to a vacuum manifold (Vac Elut 20, Agilent) and pressure was maintained at 2–5psi. The column was washed with two column-volumes of methanol, then two volumes of water. Samples were typically diluted to 20% or less methanol content and acidified to pH 3.5 and loaded onto the column at a drop rate of 1–2 drops/sec. The column was washed with two volumes of water and one volume of 50% methanol. The compounds were then eluted with one volume of 100% methanol into glass test tubes. The solution was dried down using a Thermo Reacti-Vap apparatus under compressed air and the residue was dissolved in a small amount of 100% methanol.
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