Anti ox40l pe

ELISA: The cell supernatant of feeder cells or cytotoxicity assay was collected to measure the levels of IFN-γ or Neo-2/15(Flag-tag) using their respective kits according to standard practice (R&D #DIF50C for IFN-γ, Abcam#ab125243 for Neo-2/15).
Flow cytometry: Flow cytometry was performed using the BD FACSAria system. Cells were incubated with indicated antibodies for 30 min at 4°C avoiding light. Cells were washed with DPBS and resuspended in FACS buffer before being subjected to FACS assay. Data were analyzed using FlowJo (version 10). The antibodies were from BioLegend and listed as follows: anti-CD107a-APC (328620), anti-OX40L-PE (326308), anti-CD56-PE (355504), anti-CD56-AlexaFluor488 (362518), anti-CD16-FITC (302006), anti-CD94-PE/Cy7 (305516), anti-NKG2A-APC (375108), anti-NKG2C-PE (375004), anti-NKG2D-FITC (320820), anti-KIR2DL2/L3-Percp/Cy5.5(312614), anti-KIR2DL1/S1/S3/S5-FITC (339504), anti-KIR3DL-BrilliantViolet421(312714), anti-NKp30-BrilliantViolet785 (325230), anti-NKp44-PE/Cy7 (325116), anti-NKp46-BrilliantViolet605 (137619).

Anti-CD19 APC-Cy7, anti-CD38 PerCp-Cy5.5, anti-CD4 PE-Cy7, anti-CD25 PerCP-Cy5.5, anti-CD86 APC, anti-CD80 BV421, anti-CD45RA BV605, anti-ICOS PE, anti-CD21 PE-Cy7, anti-CD80 BV421, anti-CD27 PerCp-Cy5.5, anti-PD-1 APC, anti-CD45RA APC-Cy7, anti-HLA-DR BV605, anti-CD11c AF700, anti-CD86 BV711, anti-CD95 BV650, anti-IgD BV786, anti-FCRL5 APC, anti-CXCR3 BV421, anti-CCR6 BV605, anti-CD19 BV650, anti-CD19 BV605, anti-PD-L1 BV421, anti-PD-L2 PE, anti-ICOS-L PE, anti-OX40L PE, anti-IL-10 PE, anti-IL-10 APC, anti-TNF-α APC-Cy7 (all obtained from BioLegend); anti-IgD FITC and anti-IgD PE (both from Southern Biotech); anti-CD27 PE (Dako); and anti-CD21 PE-Cy7, anti-CD69 FITC, anti-HLA-DR FITC, anti-CD27 BV605, anti-CD40 FITC, anti-CD40L PE, anti-ICAM-1 PE, anti-CXCR5 BUV395 and anti-CD27 BUV395 (all from BD Biosciences).