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17 protocols using acetic acid

1

Fabrication of Transparent Conductors using Silver Nanowire Dispersions

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EXAMPLE 1

36.5 parts by weight of a silver nanowire-containing solution (ClearOhm ink, Cambrios Technologies Corp., total amount of silver nanowires and a binder: 2.45 wt %, average diameter of silver nanowires: about 25 nm) was mixed with 63.4969 parts by weight of ultra-pure water. To the mixture, 0.003 parts by weight of acetic acid (100% pure solution, Samchun Pure Chemical Co., Ltd.) and 0.0001 parts by weight of a thiol group-compound, cysteamine (TCI), were added, followed by stirring, thereby preparing 100 parts by weight of a silver nanowire dispersion.

10 parts by weight of 100 parts by weight of the prepared silver nanowire dispersion was deposited on a base layer (polycarbonate film, WRS-148, thickness: 50 μm) by spin coating, thereby forming a layer of the silver nanowire dispersion. The layer of the silver nanowire dispersion was dried in an oven at 80° C. for 120 seconds, thereafter by spin coating 10 parts by weight of the matrix composition of Preparative Example 1 thereon and drying at an oven at 80° C. for 120 seconds and at 140° C. for 120 seconds, followed by curing through UV irradiation at a fluence of 500 mJ/cm2, thereby manufacturing a transparent conductor. Here, a capping layer having a thickness shown in Table 1 and including polyvinylpyrrolidone and cysteamine was formed on surfaces of the silver nanowires.

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2

Determination of Shikimic Acid in Artemisia absinthium

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Dried sample of A. absinthium (15 g) were extracted with ethanol under reflux system. This extract was dissolved in methanol and filtered with a syringe filter (0.45-μm). Chromatographic analysis was performed using an HPLC system (Agilent Technology 1290 Infinity II, MA, USA) equipped with a pump, auto-sampler, and UV detector with an INNO C18 column (4.6 mm × 25 cm, 5 μm). A shikimic acid standard was purchased from the Natural Product Institute of Science and Technology (Anseong, Korea). HPLC-grade solvents (methanol, water, and acetonitrile) were purchased from J. T. Baker (Phillipsburg, PA, USA). Acetic acid (99.7%) was purchased from Samchun Pure Chemicals (Pyeongtaek, Korea). Gradient elution was performed using 0.5% Acetic acid in water (A) and acetonitrile (B) at a flow rate of 1 mL/min. The initial condition was set at 95% (A) and decreased to 90% (A) after 20 min. Solvent A was further decreased to 50% until 40 min, then to 10% until 50 min, and maintained for 10 min. It was increased from 0% to 95% until 65 min. The total analysis time was 65 min. The flow rate of the mobile phase was 1 mL/min. The injection volume was 10 μL, and the detector was set at a UV absorbance of 254 nm. The column temperature was maintained at 30 °C.
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3

HPLC Analysis of Ascorbic Acid Compounds

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Ascorbic acid (drug content 97%) and calcium ascorbate (drug content 76%) were obtained from PHARMCROSS Co., Ltd (Chuncheon, Korea). Methanol for high-performance liquid chromatography (HPLC) was purchased from SKYSOLTECH® (SK Chemicals, Ulsan, Korea). Acetonitrile for sample processing was purchased from Honeywell Burdick & Jackson Labs (Muskegon, MI, USA). Acetic acid (glacial, 99.5%) was purchased from Samchun Pure Chemical (Gyeonggi-do, Korea). All other chemicals were of reagent grade and were used without further purification. The distilled and deionized water (DW) was used after sterilization.
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4

Microstructural Analysis of ZK60 Mg Alloy

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Commercial ZK60 magnesium alloy substrates with dimension of 10 mm x 10 mm x 2 mm were used in the present study. Its chemical composition (wt. %) is zinc (Zn) 5.5, zirconium (Zr) 0.49 and magnesium (Mg) balance. Prior to the coating deposition, substrates were first mechanically polished with silicon carbide papers upto 1200 grit to ensure the same surface roughness. Samples were then degreased with acetone for 10 min followed by rinsing with deionized water and dried in warm stream of air. For optical microstructure, ZK60 Mg alloy was polished further using 3 μm to 0.25 μm diamond paste. Etching solution was prepared by mixing 4.2 g of picric acid (98%, AppliChem GmbH, Korea) in a solution containing 10 ml of acetic acid (glacial, 99.5%, Samchun Pure Chemical Co., Ltd., Korea), 70 ml of ethanol (95%, Samchun Pure Chemical Co., Ltd., Korea) and 10 ml of deionized water. ZK60 Mg alloy was etched for 20–30 sec. The etched alloy was analyzed using optical microscopy (OM, BX51RF, Olympus, Tokyo, Japan) for their microstructural evaluation.
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5

Synthesis of Iron-based Nanomaterials

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Iron(ii) chloride (FeCl2·4H2O) (99%), iron(iii) chloride hexahydrate (FeCl3·6H2O) (99%), acetic acid (99.8%), hexane (99.5%), diethyl ether (99.5%), and ethanol (99.9%) were obtained from Samchun Chemical Co., Ltd. (Seoul, Republic of Korea). Ammonia solution (28%), tetraethyl orthosilicate (TEOS) (98%), (3-aminopropyl)triethoxysilane (APTES) (≥98%), and trichloroacetic acid (TCA) (99%) were obtained from Sigma Aldrich (Munich, Germany).
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6

Photosensitizer-Conjugated Nanoparticles

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Yttrium(III) chloride hexahydrate (YCl3·6H2O), erbium(III) chloride hexahydrate (ErCl3·6H2O), ytterbium(III) chloride hexahydrate (YbCl3·6H2O), ammonium fluoride (NH4F), 4-(dimethylamino) pyridine (DMAP), and triphosgene oleic acid were purchased from Aldrich Chemical Co. (Milwaukee, WI, USA). Sodium borohydride, N,N’-dicyclohexylarbodiimide (DCC), and N-hydroxysuccinimide (NHS) were obtained from Fluka (Buchs, Switzerland). 4-Hydroxy-2-butanone was purchased from TCI (Tokyo, Japan). α-Lipoic acid (LA), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), folic acid (FA), β-benzyl-L-aspartate (BLA), triethylamine (TEA), hydrazine monohydrate, PEG-bis(amine) (molecular weight: 3.350 kDa), sodium bicarbonate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Tetrahydrofuran (THF), n-hexane, benzene, N,N-dimethylformamide (DMF), methanol, chloroform, diethyl ether, dimethyl sulfoxide (DMSO), 1, 4-dioxane, acetic acid, and dichloromethane (DCM) were obtained from Samchun Pure Chemical Co., Ltd. (Gyeonggi-do, Korea). Pheophorbide a (Pha) was obtained from Frontier Scientific, Inc. (Logan, UT, USA).
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7

Phytochemical Analysis of Korean Perilla Oil

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The perilla cultivar Nulsaemi (Perilla frutescens (L.) Britton var. frutescens) was obtained from a farm in Gangwon-do, South Korea (38°03′05.9″ N 127°48′59.4″ E), and a bottle of virgin perilla oil (150 mL), made solely from raw perilla seeds, was purchased from a local grocery store “Hansallim” in Seoul, Republic of Korea. n-Hexane, ethanol, anhydrous monobasic sodium phosphate and acetic acid were acquired from Samchun Chemical Co. (Seoul, Republic of Korea). DPPH and ABTS were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Chloroform, potassium iodide, sodium thiosulfate, and soluble starch were purchased from Daejung Chemicals and Metals Co. (Daejeon, Republic of Korea). All these chemical reagents were of analytical grade. High-performance liquid chromatography (HPLC)-grade acetonitrile was obtained from Burdick & Jackson (Muskegan, MI, USA), and HPLC water was acquired from J. T Baker (Phillipsburg, NJ, USA).
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8

FRAP Assay for Antioxidant Capacity

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The FRAP solution for use in the experiment was prepared. The 300 mM acetate buffer prepared by mixing Sodium acetate trihydrate (Sigma) and acetic acid (Samchun). The above three solutions were mixed in a ratio of 10:1:1 (v/v). Also, 1.5 mL of the FRAP solution pre-warmed to 37℃ mixed with 50 μL of the casein hydrolysate fraction samples and standards. The mixture vortexed and reacted in a dark room at room temperature. The absorbance measured at 593 nm.
The results expressed in units of Ascorbic acid (Sigma) equivalent capacity in units of mM. All protein concentration of casein hydrolysate fraction samples were 200 μg/mL.
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9

Synthesis and Characterization of Ti3AlC2 Composite Materials

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Ti3AlC2 was purchased from American Elements (USA). LiF was purchased from Waco (Japan). HCl, NaCl, CaCl2·2H2O, AA, sodium metabisulfite (SM), potassium persulfate (PP), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) were purchased from Daejung Co., Ltd (Korea). TEOS and APTES were purchased from Tokyo Chemical Industry Co., Ltd (Japan). Acetic acid and MB were purchased from Samchun Pure Chemical Co., Ltd (Korea). AMPS, DMAPS, and α-Al2O3 were purchased from Sigma Aldrich (USA). Lithium chloride (LiCl) and MV were purchased from Junsei Chemical Co., Ltd (Japan). Lithium hydroxide (LiOH) monohydrate was purchased from Duksan Co., Ltd (Korea).
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10

Chitosan Microcapsules for Basil Oil Encapsulation

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In this study, the chitosan, a relatively low molecular weight of 8.0 × 10 4 , was used as a wall material for microcapsule (Chembio Co., Ltd, Korea) . Degree of deacetylation is 95%, and viscosity is 8.5 cps. the basil oil as a core material was purchased from Saeroqueens, Korea. Acetic acid (Samchun Chemicals, Inc. 2002a ) was used as a solvent for dissolving chitosan, Triton X-100 and Tween 20 (Samchun Chemicals, Inc. 2002a), Span 80 (Junsei, Japan) was used as an emulsifier. Viscosity of Triton X-100 is 240 mPa and Tween 20 is 400 mPa. Glutaraldehyde (Sigma, USA) as a crosslinking agent, N-hexane (Samchun Chemicals, Inc. 2002a) was used for washing the microcapsules, and all reagents were used more than grade 1.
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