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Phycoerythrin pe conjugated anti human cd4 antibody

Manufactured by BD

Phycoerythrin (PE)-conjugated anti-human CD4 antibody is a fluorescent-labeled monoclonal antibody that recognizes the CD4 surface marker on human T cells. It is used for the identification and enumeration of CD4+ T cells in flow cytometry applications.

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2 protocols using phycoerythrin pe conjugated anti human cd4 antibody

1

Generation of Tetracycline-inducible IFITM1 SupT1 Cells

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The tetracycline-inducible IFITM1 SupT1 cell line was generated as previously described (Lu et al., 2011 (link)). The HIV-1 BH10 proviral DNA clone was obtained from the NIH AIDS Reference and Reagent Program. The mutations Vpu34, EnvR311K, EnvG367E and g7178a were engineered using the site-directed mutagenesis kit (Stratagene). The anti-Flag and anti-β actin antibodies were purchased from Sigma, anti-tubulin antibody from Santa Cruz biotechnology, anti-HIV-1 p24 antibody from ID Lab Inc., phycoerythrin (PE)-conjugated anti-human CD4 antibody from BD Biosciences, Dylight-649-conjugated anti-Flag antibody from Rockland, FITC-conjugated anti-HIV-p24 antibody from Beckman. G418 was purchased from Invitrogen, puromycin and doxycycline from Sigma.
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2

Generation of Inducible IFITM1 SupT1 Cell Line

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The tetracycline-inducible IFITM1 SupT1 cell line was generated as previously described (Lu et al., 2011 (link)). The HIV-1 BH10 proviral DNA clone was obtained from the NIH AIDS Reference and Reagent Program. The mutations Vpu34, EnvR31IK, EnvG367E and g7178a were engineered using the site-directed mutagenesis kit (Stratagene). The anti-Flag and anti-β actin antibodies were purchased from Sigma, anti-tubulin antibody from Santa Cruz biotechnology, anti-HIV-1 p24 antibody from ID Lab Inc., phycoerythrin (PE)-conjugated anti-human CD4 antibody from BD Biosciences, Dylight-649-conjugated anti-Flag antibody from Rockland, FITC-conjugated anti-HIV-p24 antibody from Beckman. G418 was purchased from Invitrogen, puromycin and doxycycline from Sigma.
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