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Quantigene 2.0 homogenizing buffer

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Quantigene 2.0 homogenizing buffer is a solution used to prepare samples for gene expression analysis. It is designed to lyse cells and tissue samples, releasing RNA for subsequent detection and quantification.

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Lab products found in correlation

Proteinase k, by Thermo Fisher Scientific (4 mentions) Rnalater, by Merck Group (2 mentions) Bp2438, by Thermo Fisher Scientific (2 mentions) Rnalater, by Thermo Fisher Scientific (1 mentions) Anti apoe antibody, by Abcam (1 mentions)

Spelling variants of this product

Homogenizing Buffer (10 citations) Buffer II (5 citations)

4 protocols using quantigene 2.0 homogenizing buffer

1

mRNA Quantification of Mouse and NHP Brain Tissue

Cited 1 time
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Mouse brain tissue was harvested and placed in ice cold PBS (Fisher, BP2438). Brains were sliced into 300 μM sections on a vibratome. Two mm punches were taken from each section. Punches from the left side were put into RNAlater (Sigma, R0901) for mRNA quantification.
NHP brains were perfused with ice-cold PBS (Fisher, BP2438) prior to sectioning. Brains were sectioned in a brain matrix into 4 mm sections. 2 mm punches were taken from various brain regions and placed in RNAlater (Sigma, R0901) for mRNA quantification.
As described previously16 (link), tissues were lysed in Quantigene 2.0 homogenizing buffer (Invitrogen, QG0517) with proteinase K (invitrogen, 25530–049). mRNA was detected according to the Quantigene 2.0 protocol using the following probe sets: mouse HTT (SB-14150), mouse GFAP (SB-14051), mouse IBA1 (SB-3027744), mouse HPRT (SB-15463), mouse PPIB (SB-10002), mouse ApoE (SB-13611), NHP HTT (SF-10209), NHP GFAP (SF-4228397), NHP IBA-1 (SF-4214274), and NHP HPRT (SF-10356).
Alterman J.F., Godinho B.M., Hassler M.R., Ferguson C.M., Echeverria D., Sapp E., Haraszti R.A., Coles A.H., Conroy F., Miller R., Roux L., Yan P., Knox E.G., Turanov A.A., King R.M., Gernoux G., Mueller C., Gray-Edwards H.L., Moser R.P., Bishop N.C., Jaber S.M., Gounis M.J., Sena-Esteves M., Pai A.A., DiFiglia M., Aronin N, & Khvorova A. (2019). A divalent siRNA chemical scaffold for potent and sustained modulation of gene expression throughout the central nervous system. Nature biotechnology, 37(8), 884-894.
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2

mRNA Quantification of Mouse and NHP Brain Tissue

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse brain tissue was harvested and placed in ice cold PBS (Fisher, BP2438). Brains were sliced into 300 μM sections on a vibratome. Two mm punches were taken from each section. Punches from the left side were put into RNAlater (Sigma, R0901) for mRNA quantification.
NHP brains were perfused with ice-cold PBS (Fisher, BP2438) prior to sectioning. Brains were sectioned in a brain matrix into 4 mm sections. 2 mm punches were taken from various brain regions and placed in RNAlater (Sigma, R0901) for mRNA quantification.
As described previously16 (link), tissues were lysed in Quantigene 2.0 homogenizing buffer (Invitrogen, QG0517) with proteinase K (invitrogen, 25530–049). mRNA was detected according to the Quantigene 2.0 protocol using the following probe sets: mouse HTT (SB-14150), mouse GFAP (SB-14051), mouse IBA1 (SB-3027744), mouse HPRT (SB-15463), mouse PPIB (SB-10002), mouse ApoE (SB-13611), NHP HTT (SF-10209), NHP GFAP (SF-4228397), NHP IBA-1 (SF-4214274), and NHP HPRT (SF-10356).
Alterman J.F., Godinho B.M., Hassler M.R., Ferguson C.M., Echeverria D., Sapp E., Haraszti R.A., Coles A.H., Conroy F., Miller R., Roux L., Yan P., Knox E.G., Turanov A.A., King R.M., Gernoux G., Mueller C., Gray-Edwards H.L., Moser R.P., Bishop N.C., Jaber S.M., Gounis M.J., Sena-Esteves M., Pai A.A., DiFiglia M., Aronin N, & Khvorova A. (2019). A divalent siRNA chemical scaffold for potent and sustained modulation of gene expression throughout the central nervous system. Nature biotechnology, 37(8), 884-894.
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3

mRNA Quantification Using Quantigene 2.0

Cited 1 time
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mRNA quantification was performed as described (5 (link)). Briefly, tissue punches were stored in RNAlater (Invitrogen #AM7020) and homogenized in Quantigene 2.0 homogenizing buffer (Invitrogen, QG0517) with proteinase K (Invitrogen, 25530–049). mRNA was detected according to the Quantigene 2.0 protocol using the following probe sets: mouse HPRT (SB-15463), mouse PPIB (SB-10002), mouse apoE (SB-13611), human apoE (SA-10292) and human PPIB (SA-10003).
Ferguson C.M., Godinho B.M., Echeverria D., Hassler M., Vangjeli L., Sousa J., McHugh N., Alterman J., Hariharan V., Krishnamurthy P.M., Watts J., Rogaev E, & Khvorova A. (2024). A combinatorial approach for achieving CNS-selective RNAi. Nucleic Acids Research, 52(9), 5273-5284.
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4

ApoE Protein Expression and Cholesterol Quantification

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Anti-ApoE antibody (Abcam, 183597); 1:200 dilution
Anti-Vinculin antibody (Invitrogen, 700062); 1:1000 dilution
Abcam LDL and HDL cholesterol quantification kit (ab65390)
RNAlater (Invitrogen #AM7020)
Quantigene 2.0 homogenizing buffer (Invitrogen, QG0517)
Proteinase K (Invitrogen, 25530–049)
QuantiGene probesets: mouse HPRT (SB-15463), mouse PPIB (SB-10002), mouse apoE (SB-13611), human apoE (SA-10292), human PPIB (SA-10003)
WES by ProteinSimple, 16–230 kDa plate (SM-W004)
Human APOE ELISA (ab108813)
Eagle’s Minimum Essential Media (EMEM) (ATCC 302003), Fetal Bovine Serum (FBS) (Corning 35010CV)
Ferguson C.M., Godinho B.M., Echeverria D., Hassler M., Vangjeli L., Sousa J., McHugh N., Alterman J., Hariharan V., Krishnamurthy P.M., Watts J., Rogaev E, & Khvorova A. (2024). A combinatorial approach for achieving CNS-selective RNAi. Nucleic Acids Research, 52(9), 5273-5284.
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