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2 protocols using n butyl acrylate

1

Photodynamic Therapy Nanoparticle Synthesis

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Poly(ethylene glycol) methyl ether acrylate (PEGA), Ethyl 2-bromoisobutyrate (EBIB), and Tris [2-(dimethylamino) ethyl] amine were purchased from Sigma-Aldrich (Shanghai, China). n-Butyl acrylate and Isopropyl alcohol (IPA), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Chlorin e6(Ce6), and Perfluoro-15-crown-5-ether (PFCE) were obtained from Macklin (Shanghai, China). Fetal bovine serum (FBS) was purchased from Every Green (Hangzhou, China). Dulbecco’s Modified Eagle Medium (DMEM), Phosphate-Buffered Saline (PBS), and Trypsin were purchased from Solarbio (Beijing, China). 2′,7′-Dichlorodihydrofluorescein diacetate, Singlet Oxygen Sensor Green, and Hoechst 33342 were obtained from Beyotime (Shanghai, China). All of the chemicals were used as supplied without further purification.
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2

Synthesis of Soy Protein-Based Polymer

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Soy protein isolate was obtained from Shansong Biological Products Co., Ltd. (Linyi, China) (protein (%) ≥ 90). Methyl methacrylate, n-butyl acrylate (BA), ammonium persulfate, sodium dodecyl sulfate (SDS), sodium hydroxide, pentaerythritol triacrylate and bromelain (vitality, ≥300 μ/mg; relative molecular mass, approximately 33,000; light yellow powder) were obtained from Macklin (Shanghai, China). In addition, 2-(methacryloyloxy)ethyl acetoacetate (AAEM), OP-10 emulsifier, and silver nitrate were obtained from Aladdin (Shanghai, China).
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