The heads of two-day old mosquitoes were removed, fixed in 4% PFA and 0.25% Triton X-100 PBS, and sectioned using a Leica VT1200S vibratome. The anti-SYNORF1 3C11 primary monoclonal antibody (AB_528479, 1:30, Developmental Studies Hybridoma Bank (DSHB), University of Iowa) and two secondary antibodies, Alexa Fluor 488-conjugated anti-mouse IgG (#A-11029, 1:300, ThermoFisher) and anti-horseradish peroxidase 555 (anti-HRP, AB_2338959, 1:100, Jackson ImmunoResearch), were used for IHC. All imaging was conducted using a laser-scanning confocal microscope (FV3000, Olympus). Images were taken with a silicone-oil immersion 60× Plan-Apochromat objective lens (UPlanSApo, NA = 1.3) as previously described 47 (link).
Ab 528479
Manufactured by Developmental Studies Hybridoma Bank
AB_528479 is a laboratory product manufactured by Developmental Studies Hybridoma Bank. It is a piece of equipment used for scientific research and analysis, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Additional information about the intended use or interpretation of this product is not available.
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3 protocols using ab 528479
1
Immunohistochemical Analysis of Mosquito Brain
2
Immunohistochemical Analysis of Mosquito Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The heads of two-day old mosquitoes were removed, fixed in 4% PFA and 0.25% Triton X-100 PBS, and sectioned using a Leica VT1200S vibratome. The anti-SYNORF1 3C11 primary monoclonal antibody (AB_528479, 1:30, Developmental Studies Hybridoma Bank (DSHB), University of Iowa) and two secondary antibodies, Alexa Fluor 488-conjugated anti-mouse IgG (#A-11029, 1:300, ThermoFisher) and anti-horseradish peroxidase 555 (anti-HRP, AB_2338959, 1:100, Jackson ImmunoResearch), were used for IHC. All imaging was conducted using a laser-scanning confocal microscope (FV3000, Olympus). Images were taken with a silicone-oil immersion 60× Plan-Apochromat objective lens (UPlanSApo, NA = 1.3) as previously described 47 (link).
3
Mosquito Brain Immunohistochemistry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The heads of 2-day-old mosquitoes were removed, fixed in 4% paraformaldehyde (PFA) and 0.25% Triton X-100 PBS, and sectioned using a Leica VT1200S vibratome. The anti-SYNORF1 3C11 primary monoclonal antibody (AB_528479, 1:30, Developmental Studies Hybridoma Bank [DSHB], University of Iowa) and two secondary antibodies, Alexa Fluor 488-conjugated anti-mouse IgG (#A-11029, 1:300, Thermo Fisher) and anti-horseradish peroxidase 555 (anti-HRP, AB_2338959, 1:100, Jackson Immuno Research), were used for immunohistochemistry. All imaging was conducted using a laser-scanning confocal microscope (FV3000, Olympus). Images were taken with a silicone-oil immersion ×60 Plan-Apochromat objective lens (UPlanSApo, NA = 1.3) as previously described (Su et al., 2018 (link)).
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