Antibody to gapdh

Manufactured by Abcam

Sourced in United States

Antibody to GAPDH is a laboratory reagent used to detect the presence and quantify the amount of the GAPDH protein in biological samples. GAPDH is a commonly used housekeeping gene, and its expression is often used as a control in various experimental procedures.

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Lab products found in correlation

Blotto, by Santa Cruz Biotechnology (1 mentions) Ripa buffer, by Cell Signaling Technology (1 mentions)

2 protocols using antibody to gapdh

Western Blot Analysis of Duox-1 Protein

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Cells lysates were prepared in RIPA buffer (Cell signaling, Danvers, USA). Equal amounts of protein lysates were separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride (PVDF) membranes. Membranes were blocked at room temperature for 1 h with 5% blotto (Santa Cruz, Dallas, USA) followed by exposure to Duox-1 antibody (1:500 dilution in 5% blotto) overnight at 4 °C. After washing with TBS plus 1% Tween-20, membranes were incubated with 1:5000 diluted goat anti rabbit IgG-HRP antibody for 1 h at room temperature. Membranes were incubated with Pierce Western Blotting Substrate (Pierce, Rockford, USA) and imaged. Antibody to GAPDH (Abcam, Cambridge, USA) was used as a loading control.

Tian Z., Zhang H., Dixon J., Traphagen N., Wyatt T.A., Kharbanda K., Simet Chadwick S., Kolliputi N, & Allen-Gipson D.S. (2017). Cigarette Smoke Impairs A2A Adenosine Receptor Mediated Wound Repair through Up-regulation of Duox-1 Expression. Scientific Reports, 7, 44405.

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Western Blot Analysis of Autophagy Markers

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Protein samples obtained from fresh-frozen muscle biop-sies were immunoblotted as previously described
[29], Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. We performed densitomet-ric quantification from multiple gels for each experiment using ImageJ software (US National Institutes of Health). Antibodies used for western blot analysis included pri-mary antibody to microtubule-associated protein 1 light chain 3 b (LC3II) (Sigma-Aldrich, S.r.l. Milan, Italy), antibody to SQSTM1 (GSQSTM1-C) (Progen Biotechnik GmbH, Heidelberg, Germany), antibody to GAA (Sanofi Genzyme, Cambridge, MA, USA), antibody to GAPDH (Abcam, Cambridge, United Kingdom), and Horseradish Peroxidase (HRP)labelled secondary anti-mouse, anti-rabbit (GE Healthcare, Milan, Italy), and anti-guinea pig antibodies (Sigma-Aldrich).

Ripolone M., Violano R., Ronchi D., Mondello S., Nascimbeni A., Colombo I., Fagiolari G., Bordoni A., Fortunato F., Lucchini V., Saredi S., Filosto M., Musumeci O., Tonin P., Mongini T., Previtali S., Morandi L., Angelini C., Mora M., Sandri M., Sciacco M., Toscano A., Comi G.P, & Moggio M. (2018). Effects of short-to-long term enzyme replacement therapy (ERT) on skeletal muscle tissue in late onset Pompe disease (LOPD). Neuropathology and applied neurobiology, 44(5).

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