Prism v 7 or 8

Statistical differences were calculated using the Mann-Whitney test or one-way ANOVA with Tukey’s multiple comparison test with Prism v7 or 8 software (GraphPad software, La Jolla, CA).51. The data visualization in Fig. 4 was also performed using the Rstudio v1.1456, tidyverse and ggplot2 packages.

Statistical tests were performed in Prism v7 or 8 (GraphPad Software Inc.) and are described for each figure. In general, to identify outliers, the robust regression and outlier removal (ROUT) test was applied. To assess whether the data were following a normal distribution, three independent normality tests (D’Agostino and Pearson, Shapiro-Wilk, and Kolmogorov-Smirnov) were performed. If the distribution was normal, then one-way analysis of variance (ANOVA) was performed for comparisons across multiple independent samples, using Tukey’s multiple comparisons correction. If not, then Kruskal-Wallis test using Dunn’s multiple comparisons correction was used. Median with interquartile range was plotted for all behavior analysis. For RT-qPCR analysis, mean with standard SEM was plotted and an unpaired t test was performed.

Two-way ANOVA with Bonferroni’s multiple testing correction or nonlinear regression was used to analyze ELISA data from THP-1 and human and mouse microglia experiments. One-way ANOVA with Bonferroni’s multiple testing correction was used to analyze immunofluorescence quantification differences between SKF38393-treated SYN120 tg, vehicle treated SYN120 tg, SYN120 tg and WT mice. All statistical analyses were performed using GraphPad Prism v.7 or 8 (GraphPad Software, San Diego, CA, USA).