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Quantstudio 3 design and analysis software

Manufactured by Thermo Fisher Scientific

The QuantStudio 3 Design and Analysis Software is a comprehensive software solution for real-time PCR data analysis. The software provides tools for experimental design, data acquisition, and data analysis, enabling users to obtain reliable and accurate results from their real-time PCR experiments.

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2 protocols using quantstudio 3 design and analysis software

1

Quantifying SB Copy Number by qPCR

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SB copy number (CN) per cell was determined by qPCR. Genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen). CN/cell was quantified by duplex detection of the WPRE sequence, normalized to ALBUMIN, using specific primers, and detected with the TaqMan probes (Table S4). qPCR was performed using the Absolute qPCR Mix Low ROX Mix (Thermo Scientific) in a QuantStudio™ 3 Real-Time PCR System (Thermo Fisher Scientific). Results were analyzed in QuantStudio 3 Design and Analysis Software (Thermo Fisher Scientific).
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2

Quantifying Viral Copy Number via qPCR

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Viral copy number (VCN) per cell was determined by quantitative polymerase chain reaction (qPCR). Genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen). VCN per cell was quantified by duplex detection of the Psi sequence, normalized to albumin, using specific primers and detected with the TaqMan probes (table S6). qPCR was performed using the Absolute qPCR Mix Low ROX mix (Thermo Fisher Scientific) in a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific). Results were analyzed in QuantStudio 3 Design and Analysis Software (Thermo Fisher Scientific).
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