Sc 6059

Manufactured by Santa Cruz Biotechnology

Sourced in China

Sc-6059 is a laboratory instrument designed for protein analysis. It provides accurate and reliable measurements of protein concentration and purity. The core function of Sc-6059 is to facilitate the quantification and characterization of proteins, which is essential for various research and development applications.

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Lab products found in correlation

A0398, by Agilent Technologies (1 mentions) Rm 9106 s1, by Thermo Fisher Scientific (1 mentions) Sc 858, by Santa Cruz Biotechnology (1 mentions) A0452, by Agilent Technologies (1 mentions) Goat anti rabbit igg, by Agilent Technologies (1 mentions) Rabbit anti mouse igg, by Abcam (1 mentions) Affinipure goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions) Ab3748, by Abcam (1 mentions) Mg100, by R&D Systems (1 mentions) Image lab analysis, by Bio-Rad (1 mentions) Mab382, by Merck Group (1 mentions) Af 510 na, by R&D Systems (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Np 40 buffer, by Beyotime (1 mentions) Ab3242, by Merck Group (1 mentions) Anti xbp1, by Santa Cruz Biotechnology (1 mentions) Anti ho 1, by Novus Biologicals (1 mentions) Anti irf4, by Santa Cruz Biotechnology (1 mentions) Anti c myc, by Novus Biologicals (1 mentions) Anti bcl6, by Cell Signaling Technology (1 mentions)

4 protocols using sc 6059

Comprehensive Immunohistochemical Profiling

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IHC was performed with antibodies directed against B220/CD45R (RA3-6B2; R&D Systems; 1:40 dilution), CD138 (281-2; BD Biosciences; 1:50 dilution), CD3 (A0452; DAKO; 1:100 dilution), myeloperoxidase (A0398; DAKO; 1:100 dilution), Ki-67 (RM-9106-S1; Thermo Fisher Scientific; 1:200), Bcl6 (sc-858; Santa Cruz Biotechnology; 1:50), Irf4 (sc-6059; Santa Cruz Biotechnology; 1:100), and Pten (M362729-2; Agilent; 1:150 dilution). Pre-treatment of sections was conducted with EDTA for 20 min (B220, CD138, CD3, myeloperoxidase), 30 min (Ki-67, Irf4, Pten), or 40 min (Bcl6).
We used goat anti-rabbit, rabbit anti-rat, rabbit anti-goat, and rabbit anti-mouse secondary antibodies (AffiniPure Goat Anti-Rabbit IgG (H+L) [111-005-003; Jackson ImmunoResearch], AffiniPure Rabbit Anti-Rat IgG [312-005-045; Jackson ImmunoResearch], Rabbit Anti-Goat IgG [P0449; DAKO], and Rabbit Anti-Mouse IgG [ab125904; Abcam]).

Weber J., de la Rosa J., Grove C.S., Schick M., Rad L., Baranov O., Strong A., Pfaus A., Friedrich M.J., Engleitner T., Lersch R., Öllinger R., Grau M., Menendez I.G., Martella M., Kohlhofer U., Banerjee R., Turchaninova M.A., Scherger A., Hoffman G.J., Hess J., Kuhn L.B., Ammon T., Kim J., Schneider G., Unger K., Zimber-Strobl U., Heikenwälder M., Schmidt-Supprian M., Yang F., Saur D., Liu P., Steiger K., Chudakov D.M., Lenz G., Quintanilla-Martinez L., Keller U., Vassiliou G.S., Cadiñanos J., Bradley A, & Rad R. (2019). PiggyBac transposon tools for recessive screening identify B-cell lymphoma drivers in mice. Nature Communications, 10, 1415.

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Protein and Cytokine Analysis of Primary Cells

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Primary cell cultures were homogenized in NP40 buffer (Beyotime, China) supplemented with a protease inhibitor cocktail (Roche). The cell lysates were subjected to Western blotting using the anti‐IRF4 (1:500, sc‐6059, Santa Cruz), anti‐TNF‐α (1:500, AF‐510‐NA, R&D), anti‐MBP (1:500, MAB382, Chemicon), anti‐EZH2 (1:500, ab3748, Abcam), and HRP‐conjugated anti‐GAPDH (Kangcheng, Shanghai, China) antibodies. The protein bands were analyzed using Image Lab analysis (Bio‐Rad).
The TNF‐α, IL‐1β, and IGF‐1 levels in the supernatants were detected using an ELISA according to the manufacturer's instructions (EK2822, EK201B2, Multi Sciences, Hangzhou, China; MG100, R&D). The sample concentrations were calculated using an equation generated from a standard curve.

Sun D., Yu Z., Fang X., Liu M., Pu Y., Shao Q., Wang D., Zhao X., Huang A., Xiang Z., Zhao C., Franklin R.J., Cao L, & He C. (2017). LncRNA GAS5 inhibits microglial M2 polarization and exacerbates demyelination. EMBO Reports, 18(10), 1801-1816.

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Regulation of IRF4 by PGC-1α

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HEK293T cells were transfected with IRF4, PGC-1α, or both. Cell lysates were collected in RIPA buffer 48h after transfection. IRF4 protein was immunoprecipitated using anti-IRF4 antibody (sc-28696; Santa Cruz). IRF4 was western blotted using anti-IRF4 antibody (sc-6059; Santa Cruz) and anti-PGC-1α antibody (AB3242; Millipore).

Kong X., Banks A., Liu T., Kazak L., Rao R.R., Cohen P., Wang X., Yu S., Lo J.C., Tseng Y.H., Cypess A.M., Xue R., Kleiner S., Kang S., Spiegelman B.M, & Rosen E.D. (2014). IRF4 is a key thermogenic transcriptional partner of PGC-1α. Cell, 158(1), 69-83.

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Western Blot Analysis of Protein Markers

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Proteins were extracted with lysis buffer (Tri-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% NaDOC, 0.1% SDS, 1 mM Na₃VO₄, 1 mM phenylmethylsulphonyl fluoride, 1 μg ml⁻¹ pepstatin, 1 μg ml⁻¹ aprotinine and 10 μg ml⁻¹ leupeptine) for 20 min on ice, and protein amounts were quantified using Micro BCA Protein Assay Reagent (23235; Pierce Chemical Co.). Whole-cell lysates (40 μg per lane) were resolved on SDS–polyacrylamide gels, electrotransferred onto polyvinylidene difluoride membranes and examined by immunoblot analysis. The primary antibodies used were anti-PAX5 (610862; BD PharMingen), anti-BCL6 (#4242; Cell Signaling), anti-c-MYC (NB600-302C; Novus Biologicals), anti-IRF4 (sc-6059; Santa Cruz), anti-BLIMP1 (NB600-235; Novus Biologicals), anti-XBP1 (sc-7160; Santa Cruz), anti-HO-1 (NBP1-97507; Novus Biologicals), anti-AID (provided by Dr Alt) and anti-GAPDH (#2118; Cell Signaling). Full-sized scans of all western blots shown in Figs 3 and 6 and Supplementary Figs 11 and 12 are provided in Supplementary Fig. 14. Quantification of intensity was performed using an Image J (http://imagej.nih.gov/ij/).

Jang K.J., Mano H., Aoki K., Hayashi T., Muto A., Nambu Y., Takahashi K., Itoh K., Taketani S., Nutt S.L., Igarashi K., Shimizu A, & Sugai M. (2015). Mitochondrial function provides instructive signals for activation-induced B-cell fates. Nature Communications, 6, 6750.

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