Flupirtine maleate

Linopirdine dihydrochloride and flupirtine maleate were purchased from Tocris Bioscience and prepared as 10 mM stock solutions dissolved, respectively, in water or DMSO. Aliquots of the stock solutions were stored frozen and thawed once for each experiment. All other drugs were from Sigma Aldrich. 4-AP was dissolved in PSS and 0.2 ml added to 40 ml of circulating perfusate for each lung preparation.

Tetraethylammonium (TEA) a non-specific K_V channel blocker, was obtained from Sigma-Aldrich (St. Louis, MO, USA). K_V7 channel modulators, including flupirtine maleate, linopirdine dihydrochloride, and XE991 dihydrochloride, were purchased from Tocris Bioscience (Minneapolis, MN, USA). Riluzole, a glutamate release inhibitor, and CNQX and MK801, glutamate receptor antagonists, were obtained from Tocris Bioscience.
Rabbit polyclonal anti-K_V7.3 antibody was obtained from Alomone Labs (Jerusalem, Israel). Rabbit polyclonal anti-ERK1/2 and phospho-ERK1/2 antibody were purchased from Cell Signaling Technology (Danvers, MA, USA) and goat polyclonal synapsin Ia/b and goat polyclonal type 1 collagen antibody were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Horseradish peroxidase-conjugated anti-rabbit, anti-mouse antibodies were purchased from GenDEPOT (Barker, TX, USA) and the anti-goat antibody came from Santa Cruz Biotechnology, Inc.