The rs13181 SNP in the ERCC2 gene was genotyped by BGI (Beijing, China) as described15 (link) using allele-specific, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Primers designed using MassARRAY Assay Design 3.1 software (Sequenom, San Diego, CA, USA) were used to amplify fragments spanning the rs13181SNP in a 384-well ABI Veriti PCR System (Applied Biosystems, Foster City, CA, USA) in accordance with the manufacturer’s instructions. Amplification reactions (5 μL) consisted of 1 μL DNA (20 ng/μL) and 4 μL Master Mix, and reaction conditions were as follows: 94°C for 5 min, then 45 cycles of 94°C for 20 sec and 56°C for 30 sec, followed by 72°C for 1 min. Genotypes at rs13181 were analyzed using MassARRAY TYPER 4.0 software (Sequenom). Rates of successful genotyping were 99.8% (439/440) among the NPC patients and 100% (431/431) in controls.
Abi veriti pcr system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ABI Veriti PCR System is a thermal cycler designed for performing polymerase chain reaction (PCR) experiments. It features a compact design and can accommodate a variety of sample formats. The system provides temperature control and cycling functionality to enable DNA amplification and analysis workflows.
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Lab products found in correlation
2 protocols using abi veriti pcr system
1
Genotyping of ERCC2 rs13181 SNP
2
Genotyping of DC-SIGN SNPs by MALDI-TOF
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The DC-SIGN SNPs rs7252229, rs4804803, rs2287886, and rs735240 were genotyped in all subjects as described [17 (link)] using allele-specific matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, with support from BGI (Beijing, China). Fragments spanning the SNPs were amplified by PCR using primers designed with MassARRAY Assay Design 3.1 Software (Sequenom, San Diego, CA, USA). Amplifications were conducted in a 384-well ABI Veriti PCR System (Applied Biosystems) following the manufacturer's instructions. Amplification reactions (5 μL) contained 4 μL Master Mix and 1 μL DNA (20 ng/μL), and reaction conditions were as follows: 94°C for 5 min, followed by 45 cycles of 94°C for 20 sec, 56°C for 30 sec, and 72°C for 1 min. Alleles were analyzed using MassARRAY TYPER 4.0 software (Sequenom). Successful genotyping rates were 99.8% (560/561) in the control group for rs7252229, rs4804803, and rs735240, respectively, and were 99.8% (476/477) in the NPC group and 99.6% (559/561) in the control group for rs2287886.
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