Positive nucleus control probe u6

Manufactured by RiboBio

The Positive nucleus control probe U6 is a laboratory equipment designed for use in various molecular biology and cell biology applications. Its core function is to serve as a positive control for the detection and localization of U6 small nuclear RNA within the nucleus of cells. The probe provides a reliable and consistent signal to validate the performance of experimental procedures and techniques.

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Lab products found in correlation

Positive cytoplasm control probe 18s, by RiboBio (2 mentions) Fish kit, by RiboBio (1 mentions) Tcs sp8, by Leica (1 mentions) Fluorescent in situ hybridization kit, by RiboBio (1 mentions)

Close spelling variants of this product

U6 probes Control probes

2 protocols using positive nucleus control probe u6

Quantitative LINC00460 lncRNA Detection

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RNA FISH assay was performed with the Ribo FISH Kit (Ribo, China) according to the manufacturer's instructions. The lncRNA probe mix of LINC00460 and positive nucleus control probe U6 and positive cytoplasm control probe 18S were designed and purchased from Ribo. Images of cultured cells were obtained using the ZEISS Laser Scanning Confocal Microscope LSM710 with oil ×67 magnification lenses, and ZEN 2.3 software was used.

Zhu X., Li J., Ni X., Wu X., Hou X., Li Y., Li S., Zhao W, & Yin X. (2022). Pancreatic ductal adenocarcinoma cells regulated the gemcitabine‐resistance function of CAFs by LINC00460. Cancer Science, 113(11), 3735-3750.

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lncRNA Expression Profiling by RNA FISH

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RNA FISH assay was performed with Ribo Fluorescent in Situ Hybridization Kit (Ribo, China) according to manufacturer’s instructions. The lncRNA probe mix of u50535 and positive nucleus control probe U6 and positive cytoplasm control probe 18S were designed and purchased from Ribo company. The FISH results were captured by confocal instrument (Leica TCS-SP8).

Yu X., Yuan Z., Yang Z., Chen D., Kim T., Cui Y., Luo Q., Liu Z., Yang Z., Fan X., Chen D, & Wang L. (2018). The novel long noncoding RNA u50535 promotes colorectal cancer growth and metastasis by regulating CCL20. Cell Death & Disease, 9(7), 751.

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