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Dopamine hydrochloride

Manufactured by J&K Scientific
Sourced in China

Dopamine hydrochloride is a chemical compound used in scientific research and laboratory settings. It is the hydrochloride salt form of dopamine, a neurotransmitter in the human body. This product is commonly used as a standard reference material for analytical procedures, and in the study of various biological and neurological processes.

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16 protocols using dopamine hydrochloride

1

Multifunctional Polymer-Based Drug Delivery

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Sodium alginate and gelatin were purchased from Sigma–Aldrich (St. Louis, MO, USA). Dopamine hydrochloride (DA, 98%) and Tris(hydroxymethyl) aminomethane (extra pure, 99.5%) were obtained from J&K scientific Ltd. (Shanghai, China). Methacrylic acid copolymer type C (Eudragit L100-55, MAC) was from yingdemaofeng pharmaceuticals Co. Ltd. (Guangdong, China). Polycaprolactone (PCL), 1-Hydroxybenzotriazole (HOBT), and dimethylformamide (DMF) were purchased from Macklin Chemical Reagent Co. Ltd. (Shanghai, China). Doxorubicin hydrochloride (DOX·HCl), paclitaxel (PTX), and 1-Ethyl-3-(3-dimethlaminopropyl)-carbodiimide hydrochloride (EDCI) were obtained from Aladdin Chemical Reagent Co. Ltd. (Shanghai, China). Dulbecco’s modified Eagle medium (DMEM), penicillin, Trypsin-EDTA solution, and sterilized fetal bovine serum (FBS) were purchased from Solarbio (Beijing, China). Propidium iodide solution was purchased from BioLegend (San Diego, CA, USA). Mouse breast cancer (4T1) cells were obtained from Cyagen (Shanghai, China). Cell Counting kit-8 was obtained from ZomanBio (Beijing, China).
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2

Graphene-Based Dopamine Sensor Fabrication

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A PMIA staple was supplied by SRO Aramid Co., Ltd. (Huaian, China). The hollow braided rope was purchased from Tianjin Boanxin Co., Ltd. (China). Dopamine hydrochloride (DA) and tris-(hydroxymethyl)aminomethane (Tris) were purchased from J&K Scientific Ltd. (Beijing, China). GO was purchased from Suzhou Tanfeng Graphene Science and Technology Co., Ltd. (Suzhou, China). Silver nitrate (AgNO3) was bought from Tianjin Yingda Rare Chemical Reagents Factory (Tianjin, China). Dimethylacetamide (DMAc), lithium chloride (LiCl), 4-nitrophenol (4-NP) and sodium borohydride (NaBH4) were purchased from Tianjin Kermel Chemical Reagent Co., Ltd. (Tianjin, China).
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3

Electrochemical Cellulose Film Fabrication

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Dopamine hydrochloride (98%), TEMPO (99%), ammonia aqueous solution (25 to 28 wt %), NaCl (99%), acetonitrile (99.9%), tetrabutylammonium hexafluorophosphate (Bu4NPF6) (99%), and ferrocene (99.5%) were all purchased from J&K Scientific Ltd. (Beijing, China). The cellulose film was obtained from Fushun city civil affairs filter paper factory. All chemicals were freshly used without further purification. Deionized water was used in all experiments.
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4

Synthesis of SA-DA-TAA Hydrogel

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Sodium alginate (SA) was obtained from Aladdin. Dopamine hydrochloride (DA) and 3-thienylacetic acid (TAA) was obtained from J&K. 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinyl imine (NHS) and carboxymethyl chitosan (CMC) were obtained from Macklin. Sodium periodate (NaIO4) and Ferric chloride were obtained from Sigma-Aldrich. The reagents were analytically pure and not further purified.
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5

Synthesis and Characterization of Doped Silica Nanoparticles

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Standards of PHE (95%), ANT (99%), tetraethyl orthosilicate (TEOS, 98%) and polyvinylpyrrolidone (PVP) were purchased from Aladdin Industrial Co. Ltd (Shanghai, China). Dopamine hydrochloride (99%) was purchased from J&K Scientific Co. Ltd (Beijing, China). HPLC grade acetonitrile and methanol were obtained from Oceanpak Alexative Chemical Ltd (Gothenburg, Sweden). Ferric chloride hexahydrate (FeCl3·6H2O, AR), sodium borohydride (NaBH4, AR), and humic acid (HA, CP) were purchased from Guangfu Chemical Reagent Company (Tianjin, China). Sodium hydroxide (NaOH), hydrochloric acid (HCl, 37%) and sodium chloride (NaCl) were purchased from Beijing Chemical Reagent Company (Beijing, China); they were all of analytical grade and used without further purification. Standard stock solutions (100 mg l−1) of three PAH compounds were prepared in methanol prior to use. Working solutions were prepared by mixing an appropriate amount of the stock solution with ultrapure water. The concentration of organic solvent (methanol) in the final solutions was kept below 0.2% (v/v). All the standard solutions were stored at 4°C and protected from light. Ultrapure water was used during our experiments.
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6

Synthesis and Functionalization of Iron Oxide Nanoparticles

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Iron(III) acetylacetonate (Fe(acac)3, 98%), oleylamine (80~90%), benzyl ether (97%) and hexane (98%) were purchased from Aladdin Industrial Co. 1-Ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC-HCl), N,N′-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS) and a,ω-bis{2-[(3-carboxy-1-oxopropyl)amino]ethyl} poly-(ethylene glycol) (carboxylated PEG, M=2000) were purchased from Sigma Aldrich. Dopamine hydrochloride was purchased from J&K Chemical. Sodium carbonate and other organic solvents used in the syntheses were purchased from Sinopharm Chemical Reagent Co. c(RGDyK) peptide (M=620) were from GLS Biochem Ltd. All the buffers and media were from Sinopharm Chemical Reagent Co. Water was purified by Millipore Water Purification System. All chemicals were used as received without further purification. All cell lines applied in this work were originally obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and cultured under recommended conditions.
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7

Synthesis and Characterization of Modified Carbon Nanotubes

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Metronidazole (99%, analytical grade) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Carboxylic multi-walled carbon nanotubes were purchased from Aladdin Industrial Company (Shanghai, China). Dopamine hydrochloride (98%, analytical grade) was purchased from J&K Chemical (Beijing, China). Drug samples were obtained from Huayueyang Biotechnology Co., Ltd. (Beijing, China). All other reagents were of analytical grade and used without further purification. 0.1 M phosphate buffer solution (PBS) was prepared by mixing NaH2PO4 and Na2HPO4, and then adjusted to the required pH values with H3PO4 or NaOH solution. All aqueous solutions were prepared with doubly distilled water.
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8

Cellular Uptake and Lysosomal Localization Assessment

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Dopamine hydrochloride was purchased from J&K Chemical Ltd (Beijing, China). Chloroquine diphosphate was purchased from TCI chemical industry development Co., Ltd (Shanghai, China). CCK-8 kit and indocyanine green (ICG) was purchased from Dojindo Molecular Technologies (Tokyo, Japan). LysoTracker Green was obtained from Molecular Probes Inc. (Eugene, OR). Antibodies were purchased from Abcam, Inc. All other chemicals and solvents were of analytical grade commercially available unless specially mentioned otherwise. Ultrapure water (deionized (DI) water) was supplied by a Milli-Q water system (Millipore, Bedford, MA, USA).
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9

Titanium Alloy Surface Modification

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High purity titanium alloy (Ti6Al4V, 99%) sheet was purchased from Goodfellow Cambridge Ltd., Huntingdon, UK. Dopamine hydrochloride, sodium tetraborate, methacrylic anhydride, deuterium solvent and tert-butyl hydroperoxide (TBHP) were purchased from J&K Scientific Ltd., Beijing, China. Azodiisobutyronitrile (AIBN), Tris-HCl buffer and polystyrene (PS) microspheres were purchased from Aladdin Bio-Chem Technology Co., Ltd., Shanghai, China. 2-Methacryloxyethyl phosphorylcholine (MPC) was purchased by Joy-Nature Co., Nanjing, China.
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10

Graphene Synthesis via Chemical Reduction

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Hydrochloric acid (HCl, 36–38%), sulfuric acid (H2SO4, 98%), phosphoric acid (H3PO4, 85%), ethanol (CH3CH2OH, 99.5%), acetone (CH3COCH3), ethyl ether (CH3CH2OCH2CH3), hydrogen peroxide (H2O2, 30%) and potassium permanganate (KMnO4, 99.5%) were obtained from Sinopharm Chemical Reagent Beijing Co., Ltd. Natural graphite powder (325 mesh, 99%) was obtained from Qingdao Hua Tai Lubricant Sealing S&T Co., Ltd. (Qingdao, China). Sodium hydroxide (NaOH, 99%) was obtained from Xi Long Chemical Co., Ltd. Dopamine hydrochloride [(OH)2C6H3CH2CH2NH2 HCl, 99%] was obtained from J&K Scientific Ltd. The above reagents were of analytical grade, and no further purification was performed before use. Copper sheets (99.95%) (10 mm × 10 mm × 0.1 mm) were obtained from Beijing Nonferrous Metal Research Institute. Thermally conductive silicone was obtained from Qingdao Hua Tai Lubricant Sealing S&T Co., Ltd. (Qingdao, China). Deionized water was used during the experiments.
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