Hec 1a

The human EC cell lines (KLE, Ishikawa, RL95-2 and HEC-1A) were procured from Procell Life Science & Technology, Ltd (Wuhan, China). The human endometrial-stromal cell line (hESC) was procured from Otwo Biotech, Ltd (Shenzhen, China). The cells were grown in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% fetal bovine serum (FBS) at 37°C with 5% CO₂. shRNA-negative control (sh-NC), shRNA-E2F3 (sh-E2F3) and shRNA-DLEU1-1/-2 (sh-DLEU1-1/-2) were procured from Sangon Biotech, Inc (Shanghai, China). Overexpression-DLEU1 (pcDNA-DLEU1), miR-381-3p mimics and their negative controls (pcDNA-NC, miR-NC) were all procured from Ribo Biotech, Ltd (Guangzhou, China). The cells were transfected with the aforementioned agents using a Lipofectamine RNAiMAX kit (Invitrogen, Carlsbad, CA, USA) for 48 h. At 48 h after transfection, the cells were harvested to perform the following experiments.

Human endometrial cancer cell lines (Ishikawa, HEC-1A, RL95-2, HEC-1B and AN3CA) and human mononuclear cells (THP-1) were purchased from Procell Life Science & Technology Co., Ltd. All cells were cultured in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 µg/ml penicillin and 100 µg/ml streptomycin (HyClone; Cytiva) and cultured at 37˚C in 5% CO₂.

Ect/E6E7 cell line was purchased from Mingzhou biotechnology co., LTD. SiHa, HEC-1-A, ME-180, Hela cell lines were purchased from Procell life sciences LTD. All cell lines were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Rockville, MD, USA) at 37°C in a humidified incubator containing 5% CO2.